人偏肺病毒在儿童上呼吸道感染中的作用：20年经验总结
The role of human metapneumovirus in upper respiratory tract infections in children: a 20-year experience.
作者信息
Williams John V, Wang Chiaoyin K, Yang Chin-Fen, Tollefson Sharon J, House Frances S, Heck Josh M, Chu Marla, Brown Jennifer B, Lintao Linda D, Quinto Joe D, Chu David, Spaete Richard R, Edwards Kathryn M, Wright Peter F, Crowe James E
机构信息
Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
出版信息
J Infect Dis. 2006 Feb 1;193(3):387-95. doi: 10.1086/499274. Epub 2005 Dec 30.
BACKGROUND
The role that human metapneumovirus (hMPV) plays in the etiology of upper respiratory tract infections (URIs) in children over a period of many years has not been evaluated previously.
METHODS
By use of real-time reverse-transcriptase polymerase chain reaction, we retrospectively tested nasal wash (NW) specimens for hMPV that had been obtained from a cohort of 1532 infants and children with URIs who were prospectively followed for an average of 2.4 years during the period from 1982 to 2001. Virus genes were sequenced, and prospectively collected clinical data were analyzed.
RESULTS
There were 2710 visits for URIs for which routine cultures did not reveal a viral etiology. Archival NW specimens from 2384 of these visits were available. hMPV RNA was detected in 118 (5%) of 2384 specimens. The mean age of the children with hMPV infection was 20 months, and 78% of illnesses occurred from December through May. Acute otitis media (AOM) was detected in 50% of these children. hMPV circulated each year, but the numbers of isolates detected varied by year. Reinfections with both homologous and heterologous strains occurred. Four distinct genetic lineages were present over the 20 years of surveillance, with several different lineages circulating during some seasons.
CONCLUSIONS
hMPV was detected in a substantial number of children with URIs and concomitant AOM.
背景
人偏肺病毒（hMPV）在多年来儿童上呼吸道感染（URI）病因学中所起的作用此前尚未得到评估。
方法
我们使用实时逆转录聚合酶链反应，对1982年至2001年期间前瞻性随访平均2.4年的1532例患URI的婴幼儿和儿童队列所获得的鼻洗液（NW）标本进行hMPV回顾性检测。对病毒基因进行测序，并分析前瞻性收集的临床数据。
结果
有2710次URI就诊，常规培养未发现病毒病因。其中2384次就诊的存档NW标本可用。在2384份标本中的118份（5%）检测到hMPV RNA。感染hMPV的儿童平均年龄为20个月，78%的疾病发生在12月至次年5月。这些儿童中有50%检测到急性中耳炎（AOM）。hMPV每年都有传播，但每年检测到的分离株数量有所不同。发生了同源和异源毒株的再次感染。在20年的监测期间存在4个不同的基因谱系，在某些季节有几种不同的谱系同时传播。
结论
在大量患URI并伴有AOM的儿童中检测到hMPV。
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