[23-羟基丁酸对血管生成的体外抑制作用]
[In vitro inhibitory effect of 23-HBA on angiogenesis].
作者信息
Jiang Meng-jun, Yang Min, Zhou Yao-yuan, Zhang Rong-jun, Cao Guo-xian, Cai Gang-ming, Wang Guang-ji
机构信息
State Key Laboratory of Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi 214063, China.
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2006 Jan;22(1):88-91.
AIM
To study the inhibitory effect of 23-HBA on angiogenesis in vitro.
METHODS
The effect of 23-hydroxy butulinic acid (23-HBA) on the in vitro proliferation of human microcapillary endothelial cells(HMECs) was examined by sulfonylrhodamine B (SRB) assay. The effect of 23-HBA on endothelial cell migration, and tubule formation on Matrigel was also observed. The CD31 expression in HMECs was dectected by immunohistochemical staining.
RESULTS
The proliferation of HMECs was inhibited significantly by 23-HBA with IC(50) being 40.44 mg/L. 23-HBA inhibited endothelial cell migration and tubule formation in a dose-dependent manner. The expression of CD31 in HMECs was reduced after treatment with 10 mg/L 23-HBA.
CONCLUSION
23-HBA can inhibit angiogenesis in vitro, which would become a promising antiangiogenic drug.
目的
研究23 - 羟基丁酸（23 - HBA）对体外血管生成的抑制作用。
方法
采用磺酰罗丹明B（SRB）法检测23 - 羟基丁酸（23 - HBA）对人微血管内皮细胞（HMECs）体外增殖的影响。同时观察23 - HBA对内皮细胞迁移以及在基质胶上形成管腔的影响。通过免疫组织化学染色检测HMECs中CD31的表达。
结果
23 - HBA显著抑制HMECs的增殖，半数抑制浓度（IC50）为40.44 mg/L。23 - HBA以剂量依赖方式抑制内皮细胞迁移和管腔形成。用10 mg/L 23 - HBA处理后，HMECs中CD31的表达降低。
结论
23 - HBA可在体外抑制血管生成，有望成为一种有前景的抗血管生成药物。
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