Xie Fengshan, Feng Lei, Cai Weiwei, Qiu Yuyu, Liu Yanling, Li Yue, Du Bin, Qiu Liying
Laboratory of Natural Medicine, School of Pharmaceutical Science, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China.
Wuxi Medical School, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China.
Mol Med Rep. 2015 Jul;12(1):1131-6. doi: 10.3892/mmr.2015.3503. Epub 2015 Mar 18.
Angiogenesis is a major pathological component of several diseases, including traumatic vascular disease and coronary heart disease. The purpose of the present study was to determine the effects of vaccarin on endothelial cell migration and neovascularization, which are important and necessary components of wound healing. The present study investigated and confirmed neovascularization induced by vaccarin in vitro and in vivo. In vitro, the effects of vaccarin (1.08 and 2.15 µM) on proliferation, migration and tube formation of human microvascular endothelial cells (HMEC)-1 were evaluated via sulforhodamine B assay and migration and tube formation assay, respectively. Furthermore, a mouse Matrigel plus model was used to detect capillary-like tube structures in vivo. Immunohistochemistry was used to detect the protein expression of cluster of differentiation 31 (CD31), p-AKT and p-extracellular-signal-regulated kinases (Erk). Vaccarin significantly promoted HMEC-1 proliferation and migration and tube formation of HMEC-1 at a dose of 2.15 µM. In vivo, vaccarin delivered by daily oral administration significantly improved epidermal growth factor-induced angiogenesis in an intradermal inoculation mouse model. The mouse Matrigel model study also revealed that vaccarin significantly promoted neovascularization via detection of CD31 levels and enhanced protein expression of p-Akt and p‑Erk. In addition, vaccarin also promoted expression of CD31.
血管生成是包括创伤性血管疾病和冠心病在内的多种疾病的主要病理组成部分。本研究的目的是确定 vaccarin 对内皮细胞迁移和新血管形成的影响，这是伤口愈合的重要且必要的组成部分。本研究在体外和体内研究并证实了 vaccarin 诱导的新血管形成。在体外，分别通过磺酰罗丹明 B 测定法以及迁移和管形成测定法评估了 vaccarin（1.08 和 2.15 µM）对人微血管内皮细胞（HMEC）-1 增殖、迁移和管形成的影响。此外，使用小鼠基质胶加模型来检测体内的毛细血管样管结构。免疫组织化学用于检测分化簇 31（CD31）、p-AKT 和 p-细胞外信号调节激酶（Erk）的蛋白表达。Vaccarin 在 2.15 µM 的剂量下显著促进了 HMEC-1 的增殖、迁移以及 HMEC-1 的管形成。在体内，通过每日口服给药的 vaccarin 在皮内接种小鼠模型中显著改善了表皮生长因子诱导的血管生成。小鼠基质胶模型研究还表明，通过检测 CD31 水平以及增强 p-Akt 和 p-Erk 的蛋白表达，vaccarin 显著促进了新血管形成。此外，vaccarin 还促进了 CD31 的表达。
