Pradel Gabriele, Wagner Carina, Mejia Cesar, Templeton Thomas J
Research Center for Infectious Diseases, University of Würzburg, Röntgenring 11, 97070 Würzburg, Germany.
Exp Parasitol. 2006 Apr;112(4):263-8. doi: 10.1016/j.exppara.2005.11.010. Epub 2006 Jan 4.
In Plasmodium falciparum, a family of six secreted proteins having a conserved architecture of multiple adhesive domains was recently identified by genome annotation. Three of the proteins, termed PfCCp1, PfCCp2, and PfCCp3 due to a common LCCL domain, are expressed inside the gametocyte parasitophorous vacuole and released during gamete emergence, where they relocate extracellularly surrounding exflagellation complexes. In this study we show that the three PfCCp proteins are co-expressed at the gametocyte surface. Abrogation of PfCCp3 in gene disruptant parasites leads to the loss of PfCCp1 and PfCCp2 protein but not transcript, indicating a co-dependent protein expression. In view of these data we propose that the three PfCCp proteins interact during gametocytogenesis by formation of a protein complex.
在恶性疟原虫中，最近通过基因组注释鉴定出了一个由六种分泌蛋白组成的家族，这些蛋白具有多个粘附结构域的保守结构。其中三种蛋白，由于具有共同的LCCL结构域而被称为PfCCp1、PfCCp2和PfCCp3，它们在配子体寄生泡内表达，并在配子出现时释放，在那里它们重新定位到细胞外围绕着出丝复合体。在本研究中，我们表明这三种PfCCp蛋白在配子体表面共表达。基因破坏寄生虫中PfCCp3的缺失导致PfCCp1和PfCCp2蛋白丢失，但转录本未丢失，表明存在共依赖的蛋白表达。鉴于这些数据，我们提出这三种PfCCp蛋白在配子体发生过程中通过形成蛋白复合物相互作用。
