[关于mxA基因多态性与严重急性呼吸综合征易感性的病例对照研究]

[A case-control study on the mxA polymorphisms and susceptibility to severe acute respiratory syndromes].

作者信息

Wang Hong-wei, He Jing, Zhang Pan-he, Tang Fang, Wang Tian-bao, Luan Yi-he, Lv Hui, Cao Zhao-yang, Wu Xiao-ming, Zhao Qiu-min, Liu Wei, Cao Wu-chun

机构信息

Epidemiological Department of Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.

出版信息

Zhonghua Liu Xing Bing Xue Za Zhi. 2005 Aug;26(8):574-7.

PMID:16390004

Abstract

OBJECTIVE

To investigate the association between the genetic polymorphisms of myxovirus resistance 1 (MxA) gene and susceptibility to severe acute respiratory syndromes (SARS).

METHODS

A case-control study was conducted and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the T/G polymorphism at position-88 in the mxA gene promoter. Information on related factors of SARS was collected using a pre-testing questionnaire. Univariate and multivariate logistic analyses were conducted with SPSS software package.

RESULTS

Sixty-six cases and sixty-four controls were selected for the study. Comparing with GG genotype, the proportion of GT genotype were significantly higher in the case group (81.3%) than that in the control group (62.5%)) with an OR (95% CI) of 2.700 (1.208-6.037). Multivariate logistic regression analysis revealed that the significant association remained after factors as wearing masks, protection gowns and eye-protection when contacting with SARS patient etc. were adjusted with an OR (95 % CI) of 2.911 (1.027-8.250).

CONCLUSION

mxA promoter-88G/T SNP might be confered to host genetic susceptibility to SARS in Chinese Han population.

摘要

目的

探讨黏液病毒抗性1（MxA）基因的基因多态性与严重急性呼吸综合征（SARS）易感性之间的关联。

方法

进行病例对照研究，采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）检测mxA基因启动子-88位的T/G多态性。使用预测试问卷收集SARS相关因素的信息。采用SPSS软件包进行单因素和多因素逻辑分析。

结果

选择66例病例和64例对照进行研究。与GG基因型相比，病例组GT基因型的比例（81.3%）显著高于对照组（62.5%），比值比（OR，95%可信区间）为2.700（1.208 - 6.037）。多因素逻辑回归分析显示，在调整与SARS患者接触时戴口罩、穿防护服和戴眼罩等因素后，仍存在显著关联，OR（95%可信区间）为2.911（1.027 - 8.250）。