Chen Hsien-Yeh, Elkasabi Yaseen, Lahann Joerg
Departments of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
J Am Chem Soc. 2006 Jan 11;128(1):374-80. doi: 10.1021/ja057082h.
The development of generally applicable protocols for the surface modification of complex substrates has emerged as one of the key challenges in biotechnology. The use of vapor-deposited polymer coatings may provide an appealing alternative to the currently employed arsenal of surface modification methods consisting mainly of wet-chemical approaches. Herein, we demonstrate the usefulness of chemical vapor deposition polymerization for surface modification in confined microgeometries with both nonfunctionalized and functionalized poly(p-xylylenes). For a diverse group of polymer coatings, homogeneous surface coverage of different microgeometries featuring aspect ratios as high as 37 has been demonstrated based on optical microscopy and imaging X-ray photoelectron spectroscopy. In addition, height profiles of deposited polymer footprints were obtained by atomic force microscopy and imaging ellipsometry indicating continuous transport and deposition throughout the entire microchannels. Finally, the ability of reactive coatings to support chemical binding of biological ligands, when deposited in previously assembled microchannels, is demonstrated, verifying the usefulness of the CVD coatings for applications in micro/nanofluidics, where surface modifications with stable and designable biointerfaces are essential. The fact that reactive coatings can be deposited within confined microenvironments exhibits an important step toward new device architectures with potential relevance to bioanalytical, medical, or "BioMEMS" applications.
开发适用于复杂基材表面改性的通用方案已成为生物技术领域的关键挑战之一。气相沉积聚合物涂层的使用可能为目前主要由湿化学方法组成的表面改性方法提供一种有吸引力的替代方案。在此，我们展示了化学气相沉积聚合在受限微几何结构中对未官能化和官能化聚对二甲苯进行表面改性的实用性。对于多种聚合物涂层，基于光学显微镜和成像X射线光电子能谱，已证明具有高达37的纵横比的不同微几何结构能够实现均匀的表面覆盖。此外，通过原子力显微镜和成像椭圆偏振仪获得了沉积聚合物足迹的高度轮廓，表明在整个微通道中实现了连续的传输和沉积。最后，证明了反应性涂层在沉积于先前组装的微通道中时支持生物配体化学结合的能力，验证了CVD涂层在微/纳流体应用中的实用性，其中具有稳定且可设计的生物界面的表面改性至关重要。反应性涂层能够在受限微环境中沉积这一事实，朝着与生物分析、医学或“生物微机电系统”应用潜在相关的新器件架构迈出了重要一步。
