Proskurnin Mikhail A, Bendrysheva Svetlana N, Ragozina Nataliya, Heissler Stefan, Faubel Werner, Pyell Ute
M.V. Lomonosov Moscow State University, Chemistry Department, Vorob'evy Hills d. 1 str. 3 119992 GSP-3 Moscow, Russia.
Appl Spectrosc. 2005 Dec;59(12):1470-9. doi: 10.1366/000370205775142494.
The optical scheme of a near-field dual-beam mode-mismatched thermal-lens detector for capillary electrophoresis with a crossed-beam configuration employing a multimode HeCd laser (325 nm) as an excitation source was optimized. It is shown that a multimode laser can be successfully used as an excitation source in thermal lensing with minimal deviations in thermal responses from Gaussian excitation sources. An equation for diffraction thermal-lens theory for near-field measurements is deduced, and the experimental results agree with the deduced equation. The temperature rise in the capillary was estimated, and the exponential decrease of the signal with time for static conditions and low flow velocities was explained. The optimum configuration of the detector from the viewpoint of the maximum sensitivity and beam sizes was found. The detector provides a significant improvement in the detection limits for model compounds absorbing at 325 nm (nitrophenols) compared to the results obtained with a commercial absorbance detector operating at the same wavelength.
对一种用于毛细管电泳的近场双光束模式失配热透镜探测器的光学方案进行了优化，该探测器采用交叉光束配置，以多模氦镉激光器（325nm）作为激发源。结果表明，多模激光器可成功用作热透镜的激发源，其热响应与高斯激发源的偏差最小。推导了近场测量的衍射热透镜理论方程，实验结果与推导方程相符。估算了毛细管中的温度升高，并解释了静态条件和低流速下信号随时间的指数下降。从最大灵敏度和光束尺寸的角度找到了探测器的最佳配置。与在相同波长下运行的商用吸光度探测器所获得的结果相比，该探测器显著提高了对在325nm处吸收的模型化合物（硝基酚）的检测限。
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