Wernicke W, Jung G
Institut für Allgemeine Botanik, Johannes Gutenberg-Universität, Mainz, Deutschland.
Eur J Cell Biol. 1992 Feb;57(1):88-94.
The effects of oryzalin and cytochalasin B (CB) on microtubule and actin microfilament arrays and on cell shaping were investigated in developing wheat mesophyll. Excised immature leaf sections capable of differentiating were incubated with the drugs. The behavior of the cytoskeleton was monitored by fluorescence microscopy after labeling with fluorescent dyes. Brief incubation with oryzalin (40 min, 10 microM) caused disassembly of microtubules. Recovery of microtubule arrays was comparatively slow after removal of the drug. Cells failed to establish transverse cortical bands of microtubules and transverse hoops of wall reinforcement. They expanded isodiametrically rather than longitudinally without forming lobes typical of wheat mesophyll cells. Brief treatment with CB (60 min, 20 micrograms ml-1) appeared to disrupt the microfilament arrays. Filaments recovered rapidly after removal of CB, and cells were able to shape in an apparently normal fashion. Continuous incubation at comparatively low concentration of CB (4 micrograms ml-1) appeared to cause selective loss of the fine transverse cortical microfilament arrays. Cortical transverse microtubule arrays persisted, but failed to form distinct bands in the majority of the cells. Cells were able to elongate in an almost normal fashion, but no lobes were formed.
在发育中的小麦叶肉中，研究了氨磺乐灵和细胞松弛素B（CB）对微管和肌动蛋白微丝阵列以及细胞形态形成的影响。将能够分化的离体未成熟叶片切片与药物一起孵育。在用荧光染料标记后，通过荧光显微镜监测细胞骨架的行为。用氨磺乐灵短暂孵育（40分钟，10微摩尔）会导致微管解聚。去除药物后，微管阵列的恢复相对较慢。细胞未能形成微管的横向皮质带和壁增强的横向环。它们等径扩展而不是纵向扩展，没有形成小麦叶肉细胞典型的叶状结构。用CB短暂处理（60分钟，20微克/毫升）似乎会破坏微丝阵列。去除CB后，微丝迅速恢复，细胞能够以明显正常的方式形成形态。在相对较低浓度的CB（4微克/毫升）下持续孵育似乎会导致精细的横向皮质微丝阵列选择性丧失。皮质横向微管阵列持续存在，但在大多数细胞中未能形成明显的带。细胞能够以几乎正常的方式伸长，但没有形成叶状结构。
