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本文引用的文献

1
Extraction of Mycobacterium tuberculosis DNA: a question of containment.结核分枝杆菌DNA的提取：一个关于生物安全防护的问题。
J Clin Microbiol. 2005 Jun;43(6):2996-7. doi: 10.1128/JCM.43.6.2996-2997.2005.
2
Molecular characteristics and global spread of Mycobacterium tuberculosis with a western cape F11 genotype.具有西开普F11基因型的结核分枝杆菌的分子特征及全球传播
J Clin Microbiol. 2004 Feb;42(2):769-72. doi: 10.1128/JCM.42.2.769-772.2004.
3
RIDOM: comprehensive and public sequence database for identification of Mycobacterium species.RIDOM：用于鉴定分枝杆菌属物种的综合公共序列数据库。
BMC Infect Dis. 2003 Nov 11;3:26. doi: 10.1186/1471-2334-3-26.
4
The efficacy of the heat killing of Mycobacterium tuberculosis.热杀结核分枝杆菌的效力
J Clin Pathol. 2002 Oct;55(10):778-9. doi: 10.1136/jcp.55.10.778.
5
Global dissemination of the Mycobacterium tuberculosis W-Beijing family strains.结核分枝杆菌W-北京家族菌株的全球传播。
Trends Microbiol. 2002 Jan;10(1):45-52. doi: 10.1016/s0966-842x(01)02277-6.
6
Molecular evolution of Mycobacterium tuberculosis: phylogenetic reconstruction of clonal expansion.结核分枝杆菌的分子进化：克隆扩增的系统发育重建
Tuberculosis (Edinb). 2001;81(4):291-302. doi: 10.1054/tube.2001.0300.
7
Inactivation of Mycobacterium tuberculosis for DNA typing analysis.用于DNA分型分析的结核分枝杆菌灭活
J Clin Microbiol. 1999 Jul;37(7):2350-1. doi: 10.1128/JCM.37.7.2350-2351.1999.
8
Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology.通过DNA指纹图谱技术进行结核分枝杆菌菌株鉴定：标准化方法建议
J Clin Microbiol. 1993 Feb;31(2):406-9. doi: 10.1128/jcm.31.2.406-409.1993.

不影响完整性的安全结核分枝杆菌DNA提取方法。

Safe Mycobacterium tuberculosis DNA extraction method that does not compromise integrity.

作者信息

Warren Robin, de Kock Marianna, Engelke Erica, Myburgh Roseline, Gey van Pittius Nico, Victor Thomas, van Helden Paul

机构信息

DST/NRF Centre of Excellence in Biomedical Tuberculosis Research, MRC Centre for Molecular and Cellular Biology, Department of Medical Biochemistry, Faculty of Health Sciences, Stellenbosch University, Tygerberg, South Africa.

出版信息

J Clin Microbiol. 2006 Jan;44(1):254-6. doi: 10.1128/JCM.44.1.254-256.2006.

DOI:10.1128/JCM.44.1.254-256.2006
PMID:16390984
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1351970/
Abstract

Concern has been raised about the efficacy of the heat killing of mycobacteria during the isolation of DNA. We demonstrate a method that allows for the efficient killing of Mycobacterium tuberculosis without compromising DNA integrity for subsequent molecular investigation. This method reduces the risk of infection to the research scientist.

摘要

在DNA提取过程中，人们对热杀分枝杆菌的效果提出了担忧。我们展示了一种方法，该方法能够有效杀灭结核分枝杆菌，同时又不会损害DNA完整性，以便后续进行分子研究。这种方法降低了研究人员受到感染的风险。