Warren Robin, de Kock Marianna, Engelke Erica, Myburgh Roseline, Gey van Pittius Nico, Victor Thomas, van Helden Paul
DST/NRF Centre of Excellence in Biomedical Tuberculosis Research, MRC Centre for Molecular and Cellular Biology, Department of Medical Biochemistry, Faculty of Health Sciences, Stellenbosch University, Tygerberg, South Africa.
J Clin Microbiol. 2006 Jan;44(1):254-6. doi: 10.1128/JCM.44.1.254-256.2006.
Concern has been raised about the efficacy of the heat killing of mycobacteria during the isolation of DNA. We demonstrate a method that allows for the efficient killing of Mycobacterium tuberculosis without compromising DNA integrity for subsequent molecular investigation. This method reduces the risk of infection to the research scientist.