Karasuno T, Kanayama Y, Nishiura T, Nakao H, Yonezawa T, Tarui S
Second Department of Internal Medicine, Osaka University Medical School, Japan.
Eur J Immunol. 1992 Aug;22(8):2003-8. doi: 10.1002/eji.1830220808.
Castanospermine (CSP), an inhibitor of alpha-glucosidase, enhanced immunoglobulin (Ig) release in a Staphylococcus aureus Cowan I (SAC)-induced lymphocyte culture (Scand. J. Immunol. 1990. 32: 529). In a pokeweed mitogen (PWM)-human lymphocyte culture, unlike the SAC-stimulated system, CSP strongly decreased the number of IgG-, IgA- and IgM-secreting cells as well as that of Ig-bearing cells. Peripheral blood lymphocytes treated with swainsonine, a mannosidase II inhibitor, or with neuraminidase also showed a reduced response to PWM. In cross-culture experiments, only a mixture of B cells pretreated with either agent and untreated T cells showed such a suppressive effect. Adhesion was decreased between B cells treated with either agent and untreated T cells, but not between treated T cells and untreated B cells. These results demonstrate that a certain alteration in B cell membrane oligosaccharides inhibited the T cell-B cell adhesion in the PWM culture, leading to an arrest of B cell maturation. Considering that these inhibitors eventually prevent terminal sialic acid addition, the present study provides evidence that sialic acids on B cell surface oligosaccharides play a biological role in the T cell-B cell interaction.
栗精胺（CSP），一种α-葡萄糖苷酶抑制剂，在金黄色葡萄球菌考恩I株（SAC）诱导的淋巴细胞培养中可增强免疫球蛋白（Ig）的释放（《斯堪的纳维亚免疫学杂志》，1990年，第32卷，第529页）。在商陆有丝分裂原（PWM）刺激的人淋巴细胞培养中，与SAC刺激的系统不同，CSP强烈减少了分泌IgG、IgA和IgM的细胞数量以及携带Ig的细胞数量。用甘露糖苷酶II抑制剂苦马豆素或神经氨酸酶处理的外周血淋巴细胞对PWM的反应也降低。在交叉培养实验中，只有用这两种试剂之一预处理的B细胞与未处理的T细胞的混合物显示出这种抑制作用。用这两种试剂之一处理的B细胞与未处理的T细胞之间的黏附减少，但处理过的T细胞与未处理的B细胞之间的黏附没有减少。这些结果表明，B细胞膜寡糖的某种改变抑制了PWM培养中T细胞与B细胞的黏附，导致B细胞成熟停滞。鉴于这些抑制剂最终会阻止末端唾液酸的添加，本研究提供了证据，表明B细胞表面寡糖上的唾液酸在T细胞与B细胞相互作用中发挥生物学作用。
