感染单纯疱疹病毒的细胞中的RNA合成。X.病毒对称转录本及其制备的双链RNA的特性。
RNA synthesis in cells infected with herpes simplex virus. X. Properties of viral symmetric transcripts and of double-stranded RNA prepared from them.
作者信息
Jacquemont B, Roizman B
出版信息
J Virol. 1975 Apr;15(4):707-13. doi: 10.1128/JVI.15.4.707-713.1975.
Abstract
HEp-2 cells infected with herpes simplex 1 virus contain RNA transcripts capable of forming double-stranded (DS) RNA on annealing. The properties of purified DS RNA were as follows (i) DS RNA is resistant to depolymerization by RNase A or T-1 in 2 times 0.15 M NaCl, plus 0.015 M sodium citrate (SSC) but not 0.1 times SSC or following thermal denaturation. (ii) The Tm of the viral DS RNA was 100 C in 0.1 times SSC. (iii) Undenatured DS RNA does not hybridize with viral DNA: upon denaturation, excess unlabeled RNA drove 50 to 55% of labeled DNA into DNA-RNA hybrid. The kinetics of hybridization indicate that the DS RNA consists of at least two populations of transcripts arising from 29 and 26% of viral DNA and differing 40-fold in molar concentration.
摘要
感染单纯疱疹病毒1型的人喉表皮样癌细胞（HEp-2细胞）含有在退火时能够形成双链（DS）RNA的RNA转录本。纯化的DS RNA的特性如下：（i）在2倍的0.15 M NaCl加0.015 M柠檬酸钠（SSC）中，DS RNA对核糖核酸酶A或T-1的解聚具有抗性，但在0.1倍SSC中或热变性后则不然。（ii）病毒DS RNA在0.1倍SSC中的解链温度为100℃。（iii）未变性的DS RNA不与病毒DNA杂交：变性后，过量的未标记RNA使50%至55%的标记DNA形成DNA-RNA杂交体。杂交动力学表明，DS RNA至少由两个转录本群体组成，它们分别来自29%和26%的病毒DNA，摩尔浓度相差40倍。
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