Kozak M, Roizman B
Proc Natl Acad Sci U S A. 1974 Nov;71(11):4322-6. doi: 10.1073/pnas.71.11.4322.
We report two instances of selective accumulation of herpes simplex 1 RNA transcripts in different compartments of infected HEp-2 cells. In the first, transcripts derived from about 50% of the viral DNA accumulated in the nuclei of cells 8 hr after infection. However, only 40-42% of the DNA was represented in transcripts accumulating in both cytoplasm and polyribosomes. A more striking disparity in the distribution of transcripts between nuclei and cytoplasm occurred when viral infection was initiated and maintained for several hours in the absence of protein synthesis. RNA complementary to about 50% of the viral DNA accumulated in the nuclei, while transcripts derived from only about 10% of the DNA were detectable in the cytoplasm. The transcripts that were selectively transported in the presence of cycloheximide seem to be functional messenger RNA molecules, since they were found on polysomes immediately after cycloheximide reversal. In contrast, RNA retained in the nuclei during the period of cycloheximide treatment was not mobilized when protein synthesis subsequently resumed. The two instances of selective RNA transport observed during herpesvirus infection suggest that only viral transcripts competent to function in translation are exported from the nucleus.
我们报告了两例单纯疱疹病毒1型RNA转录本在感染的HEp-2细胞不同区室中选择性积累的情况。在第一例中，感染后8小时，约50%的病毒DNA衍生的转录本在细胞核中积累。然而，在细胞质和多聚核糖体中积累的转录本中，只有40 - 42%的DNA得到体现。当在缺乏蛋白质合成的情况下启动并维持病毒感染数小时时，细胞核与细胞质中转录本的分布出现了更显著的差异。与约50%的病毒DNA互补的RNA在细胞核中积累，而在细胞质中仅能检测到约10%的DNA衍生的转录本。在放线菌酮存在的情况下选择性转运的转录本似乎是功能性信使RNA分子，因为在放线菌酮作用解除后，它们立即出现在多聚核糖体上。相比之下，在放线菌酮处理期间保留在细胞核中的RNA在随后蛋白质合成恢复时并未被转运。在疱疹病毒感染期间观察到的这两例选择性RNA转运表明，只有具备翻译功能的病毒转录本才会从细胞核输出。
