Frenkel N, Roizman B, Cassai E, Nahmias A
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3784-9. doi: 10.1073/pnas.69.12.3784.
A human cervical tumor, free of detectable infectious herpes simplex 2 virus, contained a fragment comprising 39% of herpes viral DNA. Renaturation kinetics indicate that an average of 1 to 3.5 DNA fragments of herpes simplex virus are present per cell, depending on the ploidy of the cells in this particular tumor. Virus-specific sequences were found linked to highly repetitive sequences of host DNA, which reassociated under conditions designed to preclude reassociation of viral sequences. The tumor also contained RNA transcripts complementary to 5% of the viral DNA. The fraction of viral DNA template transcribed in the cervical tumor is considerably less than that transcribed in productively infected cells (50%).
一个未检测到传染性单纯疱疹病毒2型的人类宫颈肿瘤，含有一段占疱疹病毒DNA 39%的片段。复性动力学表明，根据该特定肿瘤中细胞的倍性，每个细胞平均存在1至3.5个单纯疱疹病毒DNA片段。发现病毒特异性序列与宿主DNA的高度重复序列相连，这些序列在旨在阻止病毒序列复性的条件下重新结合。该肿瘤还含有与5%的病毒DNA互补的RNA转录本。宫颈肿瘤中转录的病毒DNA模板比例远低于在产生性感染细胞中转录的比例（50%）。
