Song Desheng, Shi Bin, Xue Hua, Li Yousheng, Yu Baojun, Xu Zhe, Liu Fukun, Li Jieshou
Medical College, Nanjing University, Nanjing 210093, People's Republic of China.
Curr Microbiol. 2006 Jan;52(1):69-73. doi: 10.1007/s00284-005-0177-9. Epub 2005 Dec 26.
It has been reported that treatment with methotrexate (MTX) induces intestinal bacterial translocation; however, the definitive evidence of intestinal bacterial translocation induced by MTX has been lacking. The aim of this study was to confirm the intestinal bacterial translocation caused by MTX and to evaluate the preventive effect of granulocyte colony-stimulating factor (G-CSF) on intestinal bacterial translocation caused by MTX. Sprague-Dawley rats were treated with MTX (3.5 mg/kg) for 3 days to induce intestinal bacterial translocation; with gavaged Escherichia coli TG1 labeled with green fluorescent protein (GFP) for 2 days to track intestinal bacterial translocation; and with G-CSF (10 mug/kg) for 4 days to prevent intestinal bacterial translocation. Representative tissue specimens from the mesenteric lymph nodes, spleen, liver, and kidney were aseptically harvested for bacteria culture in ampicillin-supplemented medium. The bacteria labeled with GFP were detected in tissue specimens harvested from the rats treated with MTX but not detected in the rats that were not treated with MTX. G-CSF significantly ameliorated the situation of intestinal bacterial translocation.
据报道，甲氨蝶呤（MTX）治疗可诱导肠道细菌移位；然而，一直缺乏MTX诱导肠道细菌移位的确切证据。本研究的目的是证实MTX引起的肠道细菌移位，并评估粒细胞集落刺激因子（G-CSF）对MTX所致肠道细菌移位的预防作用。用MTX（3.5mg/kg）处理Sprague-Dawley大鼠3天以诱导肠道细菌移位；用绿色荧光蛋白（GFP）标记的大肠杆菌TG1灌胃2天以追踪肠道细菌移位；用G-CSF（10μg/kg）处理4天以预防肠道细菌移位。无菌采集肠系膜淋巴结、脾脏、肝脏和肾脏的代表性组织标本，在补充氨苄青霉素的培养基中进行细菌培养。在用MTX处理的大鼠采集的组织标本中检测到了标记有GFP的细菌，而在未用MTX处理的大鼠中未检测到。G-CSF显著改善了肠道细菌移位的情况。
