Quirino Iara Eliza Pacífico, Correia Maria Isabel Toulson Davisson, Cardoso Valbert Nascimento
School of Pharmacy, Federal University of Minas Gerais, Brazil.
Clin Nutr. 2007 Jun;26(3):335-40. doi: 10.1016/j.clnu.2006.12.007. Epub 2007 Feb 16.
BACKGROUND & AIMS: Arginine has been shown to have multiple beneficial metabolic and immunologic effects in stress situations. Supplementation of arginine has been shown to promote wound healing and intestinal mucosal recovery after trauma, ischemia or intestinal resection. Bacterial translocation has also been evaluated although with conflicting results and using different assessing techniques. Therefore, the aim of this study was to evaluate the effects of arginine on bacterial translocation in an intestinal obstruction model in rats using Escherichia coli labeled with 99mTechnetium.
Male Wistar rats (250-350 g) were randomized to receive conventional chow, diet supplemented with pure arginine or diet supplemented with an immunonutrition enteral formula, enriched with arginine, omega-3 fatty acid and RNA. After 7 days, the animals were anesthetized. Terminal ileum was isolated and a ligature was placed around it. E. coli labeled with 99mTechnetium (99mTc-E. coli) was inoculated into the intestinal lumen (terminal ileum). After 24 h, the animals were sacrificed. Blood, mesenteric lymph nodes (MLN), liver, spleen and lungs were removed for radioactivity determination.
Arginine supplementation (300 mg/day, 600 mg/day or present in the enteral formula) reduced the level of bacterial translocation when compared with the control group (p<0.05). This was shown by significantly decrease uptake of 99mTc-E. coli in blood, MLN, liver, spleen and lungs of the animals in the experimental groups (p < 0.05).
These results have shown that arginine was able to decrease bacteria translocation despite intestinal obstruction. There are several mechanisms which might explain the role of arginine and these will be the subject of future studies.
精氨酸已被证明在应激情况下具有多种有益的代谢和免疫作用。补充精氨酸已被证明可促进创伤、缺血或肠道切除术后的伤口愈合和肠黏膜恢复。尽管使用了不同的评估技术，但也对细菌移位进行了评估，不过结果相互矛盾。因此，本研究的目的是使用用99m锝标记的大肠杆菌，评估精氨酸对大鼠肠梗阻模型中细菌移位的影响。
将雄性Wistar大鼠（250 - 350克）随机分为三组，分别给予常规饲料、补充纯精氨酸的饲料或补充富含精氨酸、ω-3脂肪酸和RNA的免疫营养肠内配方的饲料。7天后，将动物麻醉。分离末端回肠并在其周围放置结扎线。将用99m锝标记的大肠杆菌（99mTc - 大肠杆菌）接种到肠腔（末端回肠）中。24小时后，处死动物。取出血液、肠系膜淋巴结（MLN）、肝脏、脾脏和肺进行放射性测定。
与对照组相比，补充精氨酸（300毫克/天、600毫克/天或肠内配方中含有的精氨酸）可降低细菌移位水平（p<0.05）。这通过实验组动物血液、MLN、肝脏、脾脏和肺中99mTc - 大肠杆菌摄取量的显著降低得以体现（p < 0.05）。
这些结果表明，尽管存在肠梗阻，精氨酸仍能够减少细菌移位。有几种机制可能解释精氨酸的作用，这些将是未来研究的主题。
