[枯草芽孢杆菌lysA基因的克隆及表达调控]

[Cloning and regulation of the expression of the lysA gene from Bacillus subtilis].

作者信息

Kal'cheva E O, Shanskaia V O, Maliuta S S

出版信息

Genetika. 1992 May;28(5):5-10.

Abstract

Cloning of Bacillus subtilis DNA fragment with the lysA gene encoding diaminopimelatecarboxylase (EC 4.1.1.20) was done. The cloned gene in poorly expressed both in Escherichia coli and in Bacillus subtilis. Some DNA sequence distant from the lysA gene seems to be necessary for full gene expression, this sequence having been not cloned together with the lysA. The sequence in needed for regulation of the expression as well.

摘要

对编码二氨基庚二酸羧化酶（EC 4.1.1.20）的枯草芽孢杆菌lysA基因的DNA片段进行了克隆。该克隆基因在大肠杆菌和枯草芽孢杆菌中表达均不佳。lysA基因完全表达似乎需要一些与其距离较远的DNA序列，而这一序列并未与lysA一起被克隆。该序列对于表达调控也是必需的。

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