Shevchenko T N, Okunev O V, Aleksieva Z M, Maliuta S S
Tsitol Genet. 1984 Jan-Feb;18(1):58-60.
Hybrid plasmids pLRS33 and pLRB4 containing Bac. subtilis genes coding lysin biosynthesis were subjected to genetical analysis. It is shown that after pLRS33- and pLRB4- transformation of E. coli strains, auxotrophic relative to lysin and diaminopimelic acid, there occurs complementation of dapA, dapB, dapC, dapD, dapE, lysA mutations by plasmid pLRS33 and of dapC, dapB, lysA mutations by plasmid pLRB4. The plasmids are studied for their influence on the level of lysin and its precurror synthesis in E. coli strains.
含有编码赖氨酸生物合成的枯草芽孢杆菌基因的杂种质粒pLRS33和pLRB4进行了遗传学分析。结果表明,在用pLRS33和pLRB4转化相对于赖氨酸和二氨基庚二酸营养缺陷的大肠杆菌菌株后,质粒pLRS33对dapA、dapB、dapC、dapD、dapE、lysA突变有互补作用,质粒pLRB4对dapC、dapB、lysA突变有互补作用。研究了这些质粒对大肠杆菌菌株中赖氨酸及其前体合成水平的影响。