CD5+ B细胞淋巴增殖性疾病中的D型细胞周期蛋白：细胞周期蛋白D1和细胞周期蛋白D2可识别诊断分组，且细胞周期蛋白D1与慢性淋巴细胞白血病中的ZAP-70表达相关。

D cyclins in CD5+ B-cell lymphoproliferative disorders: cyclin D1 and cyclin D2 identify diagnostic groups and cyclin D1 correlates with ZAP-70 expression in chronic lymphocytic leukemia.

作者信息

Meyerson Howard J, MacLennan Grayden, Husel William, Cocco Amy, Tse William, Lazarus Hillard M, Kaplan David

机构信息

Department of Pathology, Case Western Reserve University and the Ireland Cancer Center, University Hospitals of Cleveland, Cleveland, OH 44106, USA.

出版信息

Am J Clin Pathol. 2006 Feb;125(2):241-50. doi: 10.1309/7C2V-V961-P60R-MLHD.

DOI:10.1309/7C2V-V961-P60R-MLHD

PMID:16393687

Abstract

We analyzed protein expression of cyclin D1, cyclin D2, and cyclin D3 using high-resolution enzymatic amplification staining and flow cytometry in the neoplastic cells from 80 patients with CD5+ B-cell lymphoproliferative disorders. The D cyclins were expressed differentially in chronic lymphocytic leukemia (CLL), prolymphocytic leukemia (PLL), and mantle cell lymphoma (MCL) with strong staining of cyclin D1 and D2 in MCL, strong staining of cyclin D1 but weak staining of cyclin D2 in 4 of 5 PLLs, and low-level staining for both cyclins in most CLLs. No correlation between cyclin D1 and D2 and growth rates or CD38 expression was observed. However, cyclin D1 levels were significantly higher in ZAP-70+ CLL cases, although no association between ZAP-70 and cyclin D2 was detected. The results indicate that flow cytometric analysis of D cyclins may help in classification of CD5+ B-cell lymphoproliferative disorders.

摘要

我们采用高分辨率酶促扩增染色和流式细胞术，分析了80例CD5+B细胞淋巴增殖性疾病患者肿瘤细胞中细胞周期蛋白D1、细胞周期蛋白D2和细胞周期蛋白D3的蛋白表达情况。D型细胞周期蛋白在慢性淋巴细胞白血病（CLL）、幼淋巴细胞白血病（PLL）和套细胞淋巴瘤（MCL）中的表达存在差异，MCL中细胞周期蛋白D1和D2呈强染色，5例PLL中有4例细胞周期蛋白D1呈强染色而细胞周期蛋白D2呈弱染色，大多数CLL中两种细胞周期蛋白均呈低水平染色。未观察到细胞周期蛋白D1和D2与生长速率或CD38表达之间存在相关性。然而，ZAP-70阳性CLL病例中细胞周期蛋白D1水平显著更高，尽管未检测到ZAP-70与细胞周期蛋白D2之间存在关联。结果表明，对D型细胞周期蛋白进行流式细胞术分析可能有助于CD5+B细胞淋巴增殖性疾病的分类。