Henske E P, Ozelius L, Anderson M A, Kwiatkowski D J
Division of Experimental Medicine, Brigham and Women's Hospital, Boston, Massachusetts 02115.
Genomics. 1992 Jul;13(3):841-4. doi: 10.1016/0888-7543(92)90165-o.
Disease gene loci for tuberous sclerosis (TSC1), idiopathic torsion dystonia (DYT1), and nail-patella syndrome (NPS1) have been mapped by genetic linkage analysis to human chromosome 9q band 34. To create a resource for physical mapping and manipulation of this region of the genome, we have created a radiation-reduced hybrid cell line containing DNA from human 9q34 as its only human component. This cell line, E6B, has been characterized by Southern blot and PCR analysis using a panel of 9q markers and fluorescent in situ hybridization. We estimate that it contains 5 Mb of human DNA, equal to 17 cM of genetic distance, extending from AK1 to ABO on 9q34.
