Walter M A, Goodfellow P N
Department of Ophthalmology, University of Alberta, Edmonton, Canada.
Mol Biotechnol. 1995 Apr;3(2):117-28. doi: 10.1007/BF02789107.
Irradiation and fusion gene transfer (IFGT) is a technique that spans the gap between the limitations of molecular methods and somatic-cell genetics, allowing the separation of DNA fragments between 0.25 and 30 Mb in size. In conjunction with genetic linkage analysis and physical mapping techniques, IFGT provides a very useful addition to methods for cloning disease loci, and mapping chromosomes and entire genomes.
辐射与融合基因转移（IFGT）是一种跨越分子方法和体细胞遗传学局限性之间差距的技术，它能分离大小在0.25至30兆碱基之间的DNA片段。与遗传连锁分析和物理图谱绘制技术相结合，IFGT为疾病基因座克隆、染色体及整个基因组图谱绘制方法增添了非常有用的补充手段。
