Shilin S O, Rokitko P V, Romanovskaia V A
Mikrobiol Z. 2005 Sep-Oct;67(5):3-10.
Species status of the already described methylotrophic bacteria has been specified, and new isolates have been identified. The bacteria under study are classified as those from Methylobacterium genus on the basis of a set of diagnostic characteristics (they are capable to assimilate, besides methanol, other organic substances; to realize serine cycle of one-carbon compounds assimilation, to form rast-coloured colonies; they can form rosy-coloured colonies). As a result of the study of phenotypical characteristics in 14 strains of Methylobacterium and sequence-analysis of the gene 16S pRNA two strains: T-2 (=UKM B-3357) and strain ch-8 (=UKM B-3352) are identified as M. mesophilicum, strain A-5 (=UKM B-3381)--as M. extorquens; several strains were reclassified. The strain 9-17 (=UKM B-3339) is related to the species M. zatmanii (described before as M. organophilum), strain 13-17 (=UKM B-3342)--to the species M. fujisawaense (formerly--M. organophilum), K-2 (=UKM B-3351)--to the species M. fujisawaense (formerly--M. extorquens). Phenotypical characteristics differentiating the studied species of Methylobacterium are suggested. Identification of the strains of Methylobacterium genus based on sequence analysis of genes 16S pRNA is in agreement with the results obtained when studying phenotypical properties.
已描述的甲基营养型细菌的物种状态已明确，并且鉴定出了新的分离株。基于一系列诊断特征（除甲醇外，它们还能够同化其他有机物质；实现一碳化合物同化的丝氨酸循环，形成rast颜色的菌落；它们能形成玫瑰色的菌落），所研究的细菌被归类为甲基杆菌属。通过对14株甲基杆菌的表型特征研究以及对16S pRNA基因的序列分析，鉴定出两株：T-2（=UKM B-3357）和ch-8株（=UKM B-3352）为嗜温甲基杆菌，A-5株（=UKM B-3381）为扭脱甲基杆菌；对几株菌进行了重新分类。9-17株（=UKM B-3339）与扎特曼甲基杆菌（以前称为嗜有机甲基杆菌）相关，13-17株（=UKM B-3342）与藤泽甲基杆菌（以前为嗜有机甲基杆菌）相关，K-2株（=UKM B-3351）与藤泽甲基杆菌（以前为扭脱甲基杆菌）相关。提出了区分所研究的甲基杆菌属物种的表型特征。基于16S pRNA基因序列分析对甲基杆菌属菌株的鉴定与研究表型特性时获得的结果一致。
