Idris Rughia, Kuffner Melanie, Bodrossy Levente, Puschenreiter Markus, Monchy Sebastien, Wenzel Walter W, Sessitsch Angela
ARC Seibersdorf Research GmbH, Department of Bioresources, Seibersdorf, Austria.
Syst Appl Microbiol. 2006 Dec;29(8):634-44. doi: 10.1016/j.syapm.2006.01.011. Epub 2006 Feb 20.
Various pink-pigmented facultative methylotrophic (PPFM) bacteria (strains iEII3, iEIV1, iEI6, iEII1, iEIII3 iEIII4, iEIII5, iRII1, iRII2, iRIII1, iRIV1 and iRIV2) were obtained from the rhizosphere and endosphere of hyperaccumulating plant Thlaspi goesingense grown in Redschlag, Austria [R. Idris, R. Trifonova, M. Puschenreiter, W.W. Wenzel, A. Sessitsch, Bacterial communities associated with flowering plants of the Ni hyperaccumulator Thlaspi goesingense, Appl. Environ. Microbiol. 70 (2004) 2667-2677]. Due to their unexpected diversity, abundance and nickel tolerance they were further characterized by detailed 16S rRNA gene analysis, DNA-DNA hybridization, fatty acid analysis, heavy metal tolerance, screening for known Ni resistance genes and phenotypic analysis. These strains were found to exhibit different multiple heavy metal resistance characteristics to Ni, Cd, Co, Zn and Cr. On the basis of their physiological and genotypic properties, strains could be grouped with Methylobacterium extorquens and M. mesophilicum. One endophyte, strain iEII3, was found to belong to a novel species for which the name M. goesingense is proposed.
从生长在奥地利雷施拉格的超积累植物高山遏蓝菜的根际和内生菌中获得了多种粉红色色素兼性甲基营养菌（PPFM）（菌株iEII3、iEIV1、iEI6、iEII1、iEIII3、iEIII4、iEIII5、iRII1、iRII2、iRIII1、iRIV1和iRIV2）[R. 伊德里斯、R. 特里福诺娃、M. 普申赖特、W.W. 温泽尔、A. 塞西奇，与镍超积累植物高山遏蓝菜开花植物相关的细菌群落，《应用与环境微生物学》70（2004）2667 - 2677]。由于它们具有意外的多样性、丰度和镍耐受性，通过详细的16S rRNA基因分析、DNA - DNA杂交、脂肪酸分析、重金属耐受性、已知镍抗性基因筛选和表型分析对其进行了进一步表征。发现这些菌株对镍、镉、钴、锌和铬表现出不同的多重重金属抗性特征。根据它们的生理和基因型特性，这些菌株可与嗜甲基杆菌和嗜中温甲基杆菌归为一组。发现一种内生菌，即菌株iEII3，属于一个新物种，为此提出了嗜高山甲基杆菌这一名称。
