Konovalova H M, Shylin S O, Rokytko P V
Mikrobiol Z. 2007 Jan-Feb;69(1):35-41.
A detailed chromatographic and spectrophotometric analysis of carotinoids in Methylobascterium genus strains: M. extorquens B-3362, M. fujisawaense B-3365, M. mesophilicum B-3352. It has been shown that carotinoids of the studied strains are mainly represented by xanthophylls. The carotinoid common for all three strains and presented in the highest quantity was identified as oscilloxanthin. Carotene found in M. fujisawaense B-3365 and M. mesophilicum B-3352 species was identified as 3,4-didehydrolycopene. Quantitative content of carotenoids was 0.45 mg/g of dry weight for M. fujisawaense B-3365 and M. mesophilicum B-3352 and 0.23 mg/g for M. extorquens B-3362. Interspecies differences were revealed both in qualitative and quantitative content of carotinoids. However, in spite of such differences, carotinoids are similar in these three strains by Rf in thin-layer chromatography and by output time in highly efficient liquid chromatography carotinoids, and make about 80% of the total content of carotinoids.
甲基杆菌属菌株（扭脱甲基杆菌B - 3362、藤泽甲基杆菌B - 3365、嗜温甲基杆菌B - 3352）类胡萝卜素的详细色谱和分光光度分析。结果表明，所研究菌株的类胡萝卜素主要以叶黄素形式存在。在所有三个菌株中均存在且含量最高的类胡萝卜素被鉴定为振荡叶黄素。在藤泽甲基杆菌B - 3365和嗜温甲基杆菌B - 3352中发现的胡萝卜素被鉴定为3,4 - 去氢番茄红素。藤泽甲基杆菌B - 3365和嗜温甲基杆菌B - 3352的类胡萝卜素定量含量为0.45毫克/克干重，扭脱甲基杆菌B - 3362为0.23毫克/克。在类胡萝卜素的定性和定量含量方面均揭示了种间差异。然而，尽管存在这些差异，这三个菌株中的类胡萝卜素在薄层色谱中的比移值（Rf）以及高效液相色谱中类胡萝卜素的出峰时间方面相似，且占类胡萝卜素总含量的约80%。
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