Iwata M, Kiritoshi A, Roat M I, Yagihashi A, Thoft R A
Department of Ophthalmology, Eye and Ear Institute of Pittsburgh, PA 15213.
Invest Ophthalmol Vis Sci. 1992 Aug;33(9):2714-21.
The effect of recombinant human interferon gamma (IFN-gamma) on the induction of HLA class II (HLA-DR, -DP, -DQ) antigen expression on human corneal epithelial (HCE) cells was examined in different stages of culture. Primary cultures were established with limbal explants without endothelium. HCE cells in Stage 1 and Stage 2, with cells negative and positive for the 64K corneal keratin (the marker for advanced corneal epithelial differentiation), respectively, were prepared. HCE cells in both stages were treated with IFN-gamma at a concentration of 0 to 1000 U/ml for two to six days and were stained by the avidin-biotin peroxidase complex method. Class II antigens were not detected on HCE cells in either stage without IFN-gamma treatment. IFN-gamma induced three class II antigens on HCE cells in both stages in a dose- and time-dependent manner but at different levels for each antigen (DR greater than DP greater than DQ). In addition, DQ expression was related to cell differentiation, with DQ extremely rare at Stage 1 and more frequent at Stage 2 (5% vs. 20%). These findings indicate that the induction of class II antigens on HCE cells may be regulated by IFN-gamma independently for each of the antigens and that DQ induction may depend upon the differentiation of HCE cells in culture.
研究了重组人干扰素γ（IFN-γ）在培养的不同阶段对人角膜上皮（HCE）细胞上HLA II类（HLA-DR、-DP、-DQ）抗原表达的诱导作用。采用无内皮的角膜缘外植体建立原代培养。制备了处于第1阶段和第2阶段的HCE细胞，第1阶段细胞64K角膜角蛋白（晚期角膜上皮分化标志物）呈阴性，第2阶段细胞呈阳性。两个阶段的HCE细胞均用浓度为0至1000 U/ml的IFN-γ处理2至6天，并采用抗生物素蛋白-生物素过氧化物酶复合物法进行染色。在未用IFN-γ处理的任一阶段的HCE细胞上均未检测到II类抗原。IFN-γ以剂量和时间依赖性方式在两个阶段的HCE细胞上诱导出三种II类抗原，但每种抗原的诱导水平不同（DR＞DP＞DQ）。此外，DQ表达与细胞分化有关，在第1阶段极为罕见，在第2阶段更为常见（5%对20%）。这些发现表明，HCE细胞上II类抗原的诱导可能由IFN-γ对每种抗原独立调节，并且DQ的诱导可能取决于培养中HCE细胞的分化。
