Gomes J A P, Dua H S, Rizzo L V, Nishi M, Joseph A, Donoso L A
Research Division, Wills Eye Hospital, Philadelphia, PA, USA.
Br J Ophthalmol. 2004 Feb;88(2):280-5. doi: 10.1136/bjo.2003.017731.
BACKGROUND/AIMS: Peripheral blood CD8+ lymphocytes that home to mucosal surfaces express the human mucosal lymphocyte antigen (HML-1). At mucosal surfaces, including the ocular surface, only intraepithelial CD8+ lymphocytes express HML-1. These lymphocytes are retained in the intraepithelial compartment by virtue of the interaction between HML-1 and its natural ligand, E-cadherin, which is expressed on epithelial cells. The purpose of this study was to determine whether ocular surface epithelial cells (ocular mucosa) could induce the expression of human mucosal lymphocyte antigen on peripheral blood lymphocytes.
Human corneal and conjunctival epithelial cells were co-cultured with peripheral blood lymphocytes. Both non-activated and activated lymphocytes were used in the experiments. After 7 days of incubation, lymphocytes were recovered and analysed for the antigens CD8/HML-1, CD4/HML-1, CD3/CD8, CD3/CD4, CD3/CD25, CD8/CD25, and CD4/CD25 by flowcytometry.
Significant statistical differences were observed in the CD8/HML-1 expression when conjunctival epithelial cells were co-cultured with non-activated and activated lymphocytes (p = 0.04 for each) and when corneal epithelial cells were co-cultured with non-activated lymphocytes (p = 0.03). Significant statistical difference in CD4/HML-1 expression was observed only when conjunctival epithelial cells were co-cultured with activated lymphocytes (p = 0.02).
Ocular surface epithelial cells can induce the expression of human mucosal lymphocyte antigen on CD8+ (and to some extent on CD4+) lymphocytes. This may allow the retention of CD8+ and CD4+ lymphocytes within the epithelial compartment of the conjunctiva and play a part in mucosal homing of lymphocytes.
背景/目的：归巢至黏膜表面的外周血CD8 + 淋巴细胞表达人类黏膜淋巴细胞抗原（HML - 1）。在包括眼表在内的黏膜表面，仅上皮内CD8 + 淋巴细胞表达HML - 1。这些淋巴细胞通过HML - 1与其天然配体E - 钙黏蛋白（在上皮细胞表达）之间的相互作用而保留在上皮内区室。本研究的目的是确定眼表上皮细胞（眼黏膜）是否能诱导外周血淋巴细胞上人类黏膜淋巴细胞抗原的表达。
将人角膜和结膜上皮细胞与外周血淋巴细胞共培养。实验中使用未激活和激活的淋巴细胞。孵育7天后，回收淋巴细胞，并通过流式细胞术分析抗原CD8/HML - 1、CD4/HML - 1、CD3/CD8、CD3/CD4、CD3/CD25、CD8/CD25和CD4/CD25。
当结膜上皮细胞与未激活和激活的淋巴细胞共培养时，以及当角膜上皮细胞与未激活的淋巴细胞共培养时，CD8/HML - 1表达存在显著统计学差异（每组p = 0.04）和（p = 0.03）。仅当结膜上皮细胞与激活的淋巴细胞共培养时，CD4/HML - 1表达存在显著统计学差异（p = 0.02）。
眼表上皮细胞可诱导CD8 + （以及在一定程度上CD4 + ）淋巴细胞上人类黏膜淋巴细胞抗原的表达。这可能使CD8 + 和CD4 + 淋巴细胞保留在结膜的上皮区室内，并在淋巴细胞的黏膜归巢中发挥作用。
