Dadashev S Ia, Grishaeva T M, Bogdanov Iu F
Genetika. 2005 Dec;41(12):1707-13.
Earlier, using bioinformatic methods, we reported the identification of repeated DNA sequences (RS), presumably responsible for the attachment of chromatin loops to the lateral elements of synaptonemal complex in meiotic chromosomes. In the present study, consensus sequences for this class of RS were identified. It was demonstrated that at least part of these sequences belonged to the AluJb subfamily of Alu sequences. The Alu copies distribution along the major human histocompatibility complex (MHC) and their spatial separation from the sites of meiotic recombination was examined. It was demonstrated that simple sequences, like (GC/CA)n, were flanking meiotic recombination sites. A model of the RS organization in meiotic chromosome, most efficiently linking experimental data on the meiotic recombination in MHC and the in silico data on the RS localization (the coefficient of multiple correlation, r = 0.92) is suggested.
此前，我们运用生物信息学方法报告了重复DNA序列（RS）的鉴定结果，推测其与减数分裂染色体中染色质环附着于联会复合体的侧生元件有关。在本研究中，鉴定出了此类RS的共有序列。结果表明，这些序列中至少有一部分属于Alu序列的AluJb亚家族。研究了Alu拷贝沿主要组织相容性复合体（MHC）的分布及其与减数分裂重组位点的空间分离情况。结果表明，简单序列，如(GC/CA)n，位于减数分裂重组位点两侧。本文提出了减数分裂染色体中RS组织的模型，该模型能最有效地将MHC中减数分裂重组的实验数据与RS定位的计算机模拟数据联系起来（多重相关系数，r = 0.92）。
