Kiilgaard Jens F, Andersen Mads Varis Nis, Wiencke Anne K, Scherfig Erik, la Cour Morten, Tezel Tongalp H, Prause Jan U
Department of Ophthalmology, Rigshospitalet, Copenhagen, Denmark.
Acta Ophthalmol Scand. 2005 Dec;83(6):697-704. doi: 10.1111/j.1600-0420.2005.00566.x.
The purpose of this study was to evaluate the ability of different methods to induce choroidal neovascularization (CNV) in the domestic pig.
A total of 26 Danish landrace pigs was used. A sample of 22 eyes in 12 pigs underwent retinal photocoagulation with a xenon lamp, six eyes in four pigs underwent retinal photocoagulation with a diode laser, and mechanical rupture of Bruch's membrane (BM) was induced in 12 pigs following surgical debridement of the retinal pigment epithelium without damage to the neuroretina.
All 12 pigs (100%) in the group with mechanical rupture of BM developed CNV. The induced membranes were morphologically similar to CNV membranes in humans. Induced CNV was found in 13 of 22 (54%) xenon lamp-treated animals and in five of six (83%) diode laser-treated animals. The CNV in these groups was small and the morphology of the induced lesions was dominated by retinal gliosis and retinal neovascularization, probably due to a marked destruction of the neuroretina.
Surgical debridement of the retinal pigment epithelium followed by mechanical rupture of BM is a reproducible method of producing CNV in the domestic pig, whereas photocoagulation gives rise to glially derived subretinal fibrovascular membranes and primarily retinal neovascularization.
本研究旨在评估不同方法诱导家猪脉络膜新生血管（CNV）形成的能力。
共使用了26头丹麦长白猪。12头猪的22只眼睛接受了氙灯视网膜光凝，4头猪的6只眼睛接受了二极管激光视网膜光凝，12头猪在视网膜色素上皮清创术后诱导了布鲁赫膜（BM）的机械破裂，且未损伤神经视网膜。
BM机械破裂组的所有12头猪（100%）均发生了CNV。诱导形成的膜在形态上与人类的CNV膜相似。在氙灯治疗的22只动物中有13只（54%）发现了诱导性CNV，在二极管激光治疗的6只动物中有5只（83%）发现了诱导性CNV。这些组中的CNV较小，诱导病变的形态主要以视网膜胶质增生和视网膜新生血管为主，这可能是由于神经视网膜受到了明显破坏。
视网膜色素上皮清创术后进行BM机械破裂是在家猪中产生CNV的一种可重复的方法，而光凝则会导致胶质源性视网膜下纤维血管膜和主要的视网膜新生血管形成。
