Department of Biomedicine, Aarhus University, Aarhus, Denmark.
Department of Ophthalmology, Aarhus University Hospital, Aarhus, Denmark.
Transl Vis Sci Technol. 2021 Jun 1;10(7):29. doi: 10.1167/tvst.10.7.29.
The purpose of this study was to develop a porcine model for photocoagulation induced choroidal neovascularization (CNV) with high success rate and minimal thermic damage to the neuroretina.
Experimental CNV was induced by laser photocoagulation in both eyes of 16 domestic pigs. In the left eyes, photocoagulation was preceded by subretinal injection of saline to protect the neuroretina from thermic damage, whereas the right eyes were treated with photocoagulation only. The development of the CNV after 3, 7, 14, 28, and 42 days was evaluated by optical coherence tomography (OCT) scanning, fluorescein angiography, and OCT angiography, and by histology after enucleation.
From day 7 after the photocoagulation, OCT showed subretinal density in all lesions of 14 alive animals, and either fluorescein or OCT angiography confirmed CNV formation in 11 of 14 of the eyes that had received photocoagulation alone and those in which photocoagulation had been preceded by subretinal injection of saline. In all cases pretreated with subretinal saline, the neuroretina was protected from immediate thermic damage. The formation of CNVs were confirmed by histology. For both groups, the largest lesions were observed within 14 days after photocoagulation.
Injection of subretinal saline can protect the retina from thermic damage induced by retinal photocoagulation without reducing the success rate in producing experimental CNV. The effect of interventional studies aimed at reducing photocoagulation induced experimental CNV in pigs can be evaluated within 2 weeks after photocoagulation.
This model provides a fundament to develop and evaluate novel treatment methods for neovascular retinal diseases.
本研究旨在建立一种成功率高且对神经视网膜热损伤极小的猪光凝诱导脉络膜新生血管（CNV）模型。
在 16 只家猪的双眼进行激光光凝以诱导实验性 CNV。左眼在光凝前行视网膜下注射生理盐水以保护神经视网膜免受热损伤，而右眼仅行光凝。在光凝后第 3、7、14、28 和 42 天，通过光学相干断层扫描（OCT）扫描、荧光素血管造影和 OCT 血管造影以及眼球摘除法进行 CNV 发展评估。
在光凝后第 7 天，所有 14 只存活动物的 OCT 均显示视网膜下密度，单独行光凝的 14 只眼中的 11 只以及先行视网膜下注射生理盐水的眼中，荧光素或 OCT 血管造影均证实 CNV 形成。所有预先用视网膜下生理盐水处理的动物，其神经视网膜均免受即刻热损伤。通过组织学证实了 CNV 的形成。对于两组，在光凝后 14 天内观察到最大的病变。
视网膜下注射生理盐水可保护视网膜免受光凝引起的热损伤，而不降低产生实验性 CNV 的成功率。可以在光凝后 2 周内评估旨在减少猪光凝诱导实验性 CNV 的干预性研究的效果。
杨宁
