Fioravanti A, Benetti D, Coppola G, Collodel G
Department of Clinical Medicine and Immunological Sciences, Rheumatology Unit, University of Siena, Siena, Italy.
Clin Exp Rheumatol. 2005 Nov-Dec;23(6):847-53.
To investigate the effect of continuous high hydrostatic pressure on the cell ultrastructure and cytoskeleton of cultivated normal and osteoarthritic (OA) human chondrocytes.
The effects of continuous hydrostatic pressure (24 MPa) for 3 hrs on normal and OA chondrocytes were assessed by transmission electron microscopy (TEM), scanning electron microscopy (SEM) and immunofluorescence microscopy (IF).
Structural differences at the nuclear, cytoplasmic and cytoskeletal levels were observed between normal and OA chondrocytes. Continuous high hydrostatic pressure severely altered normal chondrocytes that became similar in structural organization to OA chondrocytes and further reduced the number of cell organelles involved in the synthesis of collagen and proteoglycans. IF showed major changes in the distribution of actin and tubulin after pressurization in normal and OA chondrocytes.
The results confirm the major role of pressure on chondrocyte ultrastructure. Continuous high hydrostatic pressure caused structural alterations in normal chondrocytes, which obtained similar, if not identical, characteristics to those typical of osteoarthritic chondrocytes.
研究持续高静水压力对培养的正常及骨关节炎（OA）人软骨细胞的细胞超微结构和细胞骨架的影响。
通过透射电子显微镜（TEM）、扫描电子显微镜（SEM）和免疫荧光显微镜（IF）评估持续静水压力（24兆帕）3小时对正常和OA软骨细胞的影响。
在正常和OA软骨细胞之间观察到核、细胞质和细胞骨架水平的结构差异。持续高静水压力严重改变了正常软骨细胞，使其结构组织变得与OA软骨细胞相似，并进一步减少了参与胶原蛋白和蛋白聚糖合成的细胞器数量。IF显示正常和OA软骨细胞加压后肌动蛋白和微管蛋白的分布发生了重大变化。
结果证实了压力对软骨细胞超微结构的主要作用。持续高静水压力导致正常软骨细胞发生结构改变，使其获得了与骨关节炎软骨细胞典型特征相似（即便不完全相同）的特征。
