Lee C Y, Ryan R J
J Clin Endocrinol Metab. 1975 Feb;40(2):228-33. doi: 10.1210/jcem-40-2-228.
A sensitive radioreceptor assay capable of detecting 100 pg of human chorionic gonadotropin has been developed using ovarian receptors and (125I)hCG. The dose-response curves of a series of urine and serum samples from pregnant women were linear and parallel to standard curves of 2nd International Standard hCG and purified hCG when logit of percent bound was plotted against log dose. Potency estimates of pregnancy sera, measured by radioreceptor assays, were approximately 2 times greater than those determined by radioimmunoassays. Examination of hCG concentrations in the urine of pregnant and nonpregnant women, using a receptor assay, showed good correlation with the result obtained with a standard agglutination pregnancy test. This readioreceptor assay, which can be made as sensitive as radioimmunoassay, provides a rapid and simple method for the measurement of biologically active hCG and LH.
利用卵巢受体和（125I）人绒毛膜促性腺激素（hCG）开发了一种能够检测100 pg人绒毛膜促性腺激素的灵敏放射受体测定法。当绘制结合百分比的对数与对数剂量的关系图时，一系列孕妇尿液和血清样本的剂量反应曲线呈线性，且与第二国际标准hCG和纯化hCG的标准曲线平行。通过放射受体测定法测得的妊娠血清效价估计值比放射免疫测定法测定的结果大约高2倍。使用受体测定法检测孕妇和非孕妇尿液中的hCG浓度，结果与标准凝集妊娠试验得到的结果具有良好的相关性。这种放射受体测定法可达到与放射免疫测定法相同的灵敏度，为测量生物活性hCG和促黄体生成素（LH）提供了一种快速简便的方法。
