Roma Andres A, Magi-Galluzzi Cristina, Kral Melinda A, Jin Tao T, Klein Eric A, Zhou Ming
Department of Anatomic Pathology, Cleveland Clinic Foundation, Cleveland, OH 44195, USA.
Mod Pathol. 2006 Mar;19(3):392-8. doi: 10.1038/modpathol.3800546.
Lymphangiogenesis, detected by antibodies specific for lymphatic endothelial cells, has been associated with regional lymph node metastases and poor prognosis in carcinomas of head and neck, breast and uterine cervix, but remains largely uninvestigated in prostate adenocarcinoma. We evaluated the lymphatic vessel density and lymphatic vessel invasion by prostate cancer cells in the intratumoral, peritumoral and normal prostate tissue compartments in cancer-bearing prostate glands and correlated them with lymph node metastases, Gleason score and other pathological parameters. Lymphatic vessels were detected by immunohistochemical stain using an antibody specific for the lymphatic endothelial cells (clone D2-40) on 33 radical prostatectomies. In all, 26 patients had lymph node dissection, and 14 of them had lymph node metastasis. The lymphatic vessel density and lymphatic vessel invasion were then recorded for each of the three compartments microscopically. Lymphatic vessel density in the intratumoral, peritumoral and normal prostate compartments was 0.91+/-0.80, 1.54+/-0.68 and 1.58+/-0.96/mm2, respectively. The intratumoral lymphatic vessel density was significantly lower than that of the peritumoral and normal prostate compartments, and the latter two were not significantly different. The lymphatic vessel density of the three compartments was not significantly different between cases with and without lymph node metastasis. The peritumoral lymphatic vessel density correlated inversely with the Gleason score. Lymphatic vessel invasion was present in significantly higher percentage of cases with lymph node metastasis (9/14, 62.3%), as compared to those without lymph node metastasis (1/12, 8.3%, P<0.01). The peritumoral lymphatic vessel invasion had a better correlation with the presence of lymph node metastases than intratumoral lymphatic vessel invasion. There is no evidence of lymphangiogenesis in prostate adenocarcinoma. Peritumoral lymphatic vessel invasion correlates with regional lymph node metastases, suggesting that the peritumoral lymphatic vessels are functionally important and identification of lymphatic vessel invasion in this compartment implies a high probability of regional lymph node metastases.
通过针对淋巴管内皮细胞的特异性抗体检测到的淋巴管生成，已与头颈部癌、乳腺癌和宫颈癌的区域淋巴结转移及不良预后相关，但在前列腺腺癌中仍基本未被研究。我们评估了荷癌前列腺腺体瘤内、瘤周和正常前列腺组织区域中前列腺癌细胞的淋巴管密度和淋巴管侵犯情况，并将其与淋巴结转移、 Gleason评分及其他病理参数相关联。使用针对淋巴管内皮细胞的特异性抗体（克隆D2-40）通过免疫组织化学染色对33例根治性前列腺切除术标本检测淋巴管。总共26例患者进行了淋巴结清扫，其中14例有淋巴结转移。然后在显微镜下记录三个区域各自的淋巴管密度和淋巴管侵犯情况。瘤内、瘤周和正常前列腺区域的淋巴管密度分别为0.91±0.80、1.54±0.68和1.58±0.96/mm²。瘤内淋巴管密度显著低于瘤周和正常前列腺区域，而后两者无显著差异。有无淋巴结转移的病例之间，三个区域的淋巴管密度无显著差异。瘤周淋巴管密度与Gleason评分呈负相关。与无淋巴结转移的病例（1/12，8.3%，P<0.01）相比，有淋巴结转移的病例中淋巴管侵犯的比例显著更高（9/14，62.3%）。瘤周淋巴管侵犯与淋巴结转移的相关性比瘤内淋巴管侵犯更好。没有证据表明前列腺腺癌中有淋巴管生成。瘤周淋巴管侵犯与区域淋巴结转移相关，提示瘤周淋巴管在功能上很重要，在此区域识别淋巴管侵犯意味着区域淋巴结转移的可能性很高。
