Potier Noelle, Rogniaux Hélène, Chevreux Guillaume, Van Dorsselaer Alain
Laboratoire de Spéctrometrie de Masse Bio-Organique, Strasbourg, France.
Methods Enzymol. 2005;402:361-89. doi: 10.1016/S0076-6879(05)02011-2.
The objective of this chapter is to show the general mass spectrometry (MS)-based strategies that can be used to retrieve information regarding protein-metal and protein-ligand noncovalent complexes. Indeed, when using carefully controlled conditions in the atmospheric pressure-vacuum interface of the mass spectrometer, and when sample preparation is optimized, it is possible to preserve large specific multiprotein-metal-ligand noncovalent complexes during MS analysis. Examples describing the possibilities of electrospray ionization MS (ESI-MS) are shown. For instance, it can be used to probe cooperativity in the binding of a ligand or a metal to a protein or may constitute a new methodology for a more rational approach for drug discovery and for human genome annotation. Thanks to its ability to directly give information on stoichiometry or dynamics of the interactions formed in solution, MS offers new possibilities to tackle more and more various applications.
本章的目的是展示基于质谱（MS）的通用策略，这些策略可用于获取有关蛋白质 - 金属和蛋白质 - 配体非共价复合物的信息。实际上，当在质谱仪的大气压 - 真空接口中使用精心控制的条件，并且样品制备得到优化时，在质谱分析过程中有可能保留大型特定多蛋白 - 金属 - 配体非共价复合物。文中展示了描述电喷雾电离质谱（ESI - MS）可能性的示例。例如，它可用于探测配体或金属与蛋白质结合中的协同作用，或者可能构成一种新方法，用于以更合理的方式进行药物发现和人类基因组注释。由于质谱能够直接提供有关溶液中形成的相互作用的化学计量或动力学信息，因此它为解决越来越多的各种应用提供了新的可能性。
