Mizoguchi S, Takahashi K, Takeya M, Naito M, Morioka T
Second Department of Pathology, Kumamoto University Medical School, Japan.
J Leukoc Biol. 1992 Jul;52(1):52-61. doi: 10.1002/jlb.52.1.52.
An antirat monoclonal antibody (mAb) against nonlymphoid dendritic cells, RED-1, was produced using epidermal Langerhans cells (LCs) as the immunogen. This mAb reacted mainly with the LCs and indeterminate dendritic cells (ICs), interdigitating cells in the T cell areas of lymphoid tissues, and monocyte/macrophages in various organs and tissues of adult rats. In the epidermal sheets prepared from adult rats, it specifically recognized the cell surface antigen(s) present on LCs and ICs. In the fetal rat skin, primitive or fetal macrophages migrated into the epidermis and expressed RED-1 at fetal day 17. With advance of gestation, RED-1-positive cells increased, started expressing Ia antigens at fetal day 18, and subsequently differentiated into dendritic cells. Most of them showed Ia expression by fetal day 20 and differentiated into LCs within a few days after birth. The labeling index of 5-bromo-2'-deoxyuridine in RED-1-positive cells was 18% at fetal day 17 and decreased to 5 to 6% in the postnatal period. These results imply that proliferative capacity of RED-1-positive cells is important for the formation and expansion of the IC population in the fetal stage and for the survival of LCs in the postnatal period.
利用表皮朗格汉斯细胞（LCs）作为免疫原，制备了一种抗非淋巴树突状细胞的抗大鼠单克隆抗体（mAb）RED-1。该单克隆抗体主要与LCs、不确定树突状细胞（ICs）、淋巴组织T细胞区的交错突细胞以及成年大鼠各种器官和组织中的单核细胞/巨噬细胞发生反应。在成年大鼠制备的表皮片中，它能特异性识别LCs和ICs上存在的细胞表面抗原。在胎鼠皮肤中，原始或胎儿巨噬细胞在胚胎第17天迁移到表皮并表达RED-1。随着妊娠进展，RED-1阳性细胞增加，在胚胎第18天开始表达Ia抗原，随后分化为树突状细胞。到胚胎第20天，大多数细胞显示Ia表达，并在出生后几天内分化为LCs。RED-1阳性细胞中5-溴-2'-脱氧尿苷的标记指数在胚胎第17天为18%，在出生后降至5%至6%。这些结果表明，RED-1阳性细胞的增殖能力对于胎儿期IC群体的形成和扩展以及出生后LCs的存活很重要。
