Drevets D A, Canono B P, Campbell P A
Department of Medicine, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206.
J Leukoc Biol. 1992 Jul;52(1):70-9. doi: 10.1002/jlb.52.1.70.
Listeria monocytogenes is a facultative intracellular bacterium that escapes phagocytic vesicles and replicates in the cytoplasm, where it becomes coated with F-actin. Macrophages, important anti-Listeria effector cells, are heterogeneous in their ability to kill Listeria. Complement receptor type 3 (CR3) mediates most phagocytosis of Listeria by listericidal macrophages. Experiments described here tested whether nonlistericidal macrophages also phagocytosed Listeria through CR3 and whether the ability of Listeria to escape into the cytoplasm correlated with lack of listericidal activity. We show here that CR3 mediated an average of 66% of the phagocytosis of serum-opsonized Listeria by listericidal peptone-elicited macrophages but only 35% by nonlistericidal thioglycolate-elicited macrophages. In thioglycolate-elicited macrophages, most Listeria were cytoplasmic and actin coated, whereas in peptone-elicited macrophages most were retained in the phagosome. These results indicate that listericidal and nonlistericidal macrophages phagocytose Listeria through different receptors and that nonlistericidal macrophages allow Listeria to escape into the cytoplasm.
单核细胞增生李斯特菌是一种兼性胞内细菌，它能逃离吞噬小泡并在细胞质中复制，在细胞质中它会被F - 肌动蛋白包裹。巨噬细胞是重要的抗李斯特菌效应细胞，其杀灭李斯特菌的能力具有异质性。3型补体受体（CR3）介导了具有杀菌作用的巨噬细胞对李斯特菌的大部分吞噬作用。本文所述实验测试了非杀菌性巨噬细胞是否也通过CR3吞噬李斯特菌，以及李斯特菌逃入细胞质的能力是否与缺乏杀菌活性相关。我们在此表明，CR3介导了经蛋白胨诱导产生的具有杀菌作用的巨噬细胞对血清调理的李斯特菌平均66%的吞噬作用，但经巯基乙酸盐诱导产生的非杀菌性巨噬细胞对其吞噬作用仅为35%。在经巯基乙酸盐诱导产生的巨噬细胞中，大多数李斯特菌位于细胞质中且被肌动蛋白包裹，但在经蛋白胨诱导产生的巨噬细胞中，大多数李斯特菌被保留在吞噬小体中。这些结果表明，具有杀菌作用和非杀菌作用的巨噬细胞通过不同受体吞噬李斯特菌，并且非杀菌性巨噬细胞允许李斯特菌逃入细胞质。
