Breen E J, Eliott S, Vardy P H, White A, Williams K L
School of Biological Sciences, Macquarie University, Sydney, N.S.W., Australia.
J Exp Zool. 1992 Jun 1;262(3):299-306. doi: 10.1002/jez.1402620310.
Time-lapse video light microscopy was used to study the emergence and maturation of the migratory slug from a D. discoideum aggregate. The anterior part, the tip of this simple multicellular organism, establishes migration prior to the definition of the rear, and hence the length of the slug. It was found that newly formed slugs of wild-type strain WS380B can reach lengths greater than 1 cm, yet mature slugs of this strain are rarely longer than 2-3 mumm. Often the tip extended out of the aggregation mound upon an arching pillar of cells. After the tip first touched the substratum, it commenced migration with a rapid succession of movement steps. Here we show that at the initiation of migration, a differential rate of cell movement along the developing slug axis results in a series of complicated changes, before the stable and mature shape of the slug is formed. Our results lead to new conclusions about D. discoideum slug formation and shape maintenance. Evidence is presented for regulation of slug length.
采用延时视频光学显微镜研究了盘基网柄菌聚集体中迁移蛞蝓体的出现和成熟过程。这个简单的多细胞生物体前端，即其顶端，在后端确定之前就开始迁移，从而确定了蛞蝓体的长度。研究发现，野生型菌株WS380B新形成的蛞蝓体长度可超过1厘米，但该菌株成熟蛞蝓体很少长于2 - 3毫米。蛞蝓体顶端常常通过细胞构成的拱形支柱伸出聚集丘。顶端首次接触基质后，便以一系列快速的移动步骤开始迁移。我们在此表明，在迁移开始时，沿着发育中的蛞蝓体轴的细胞移动速率差异会导致一系列复杂变化，之后才形成稳定且成熟的蛞蝓体形状。我们的研究结果得出了关于盘基网柄菌蛞蝓体形成和形状维持的新结论。文中提供了关于蛞蝓体长度调控的证据。
