Breen E J, Williams K L
School of Biological Sciences, Macquarie University, Sydney, NSW, Australia.
Microbiology (Reading). 1994 May;140 ( Pt 5):1241-52. doi: 10.1099/13500872-140-5-1241.
A digital image analysis system for extracting motion information from time-varying digital light microscopy images is presented. This system is then used to map out the movement profile of the surface layer of cells in contact with the substratum through the extracellular matrix (ECM) of the migrating Dictyostelium discoideum slug. From digital high magnification light microscopy images, the morphology of moving cells within the tail region of a young migrating wild-type WS380B slug is described, and compared with the morphology of streaming D. discoideum cells. It is shown that: (i) when the migrating tip of the slug touches the agar substrate, cells in the anterior ventral surface layer of the tip region slow dramatically; (ii) overall cell movement in the ventral surface layer of the migrating D. discoideum slug is slower than the movement of the slug as a whole; and (iii) in less than 10% of cases a wave of movement (groups of cells synchronously slowing down and then accelerating forward) propagates down the slug axis at approx. 1.2 microns s-1. The time interval between waves may be related to the time interval between tip-to-substratum contact that is periodically re-established during normal WS380B slug migration after each aerial projection of the tip.
本文介绍了一种用于从随时间变化的数字光学显微镜图像中提取运动信息的数字图像分析系统。该系统随后被用于绘制通过迁移的盘基网柄菌蛞蝓的细胞外基质（ECM）与基质接触的细胞表层的运动轮廓。从数字高倍光学显微镜图像中，描述了年轻迁移的野生型WS380B蛞蝓尾部区域内移动细胞的形态，并与流动的盘基网柄菌细胞的形态进行了比较。结果表明：（i）当蛞蝓的迁移尖端接触琼脂底物时，尖端区域前腹表层的细胞显著减慢；（ii）迁移的盘基网柄菌蛞蝓腹表层的整体细胞运动比蛞蝓整体的运动慢；（iii）在不到10%的情况下，一波运动（细胞群同步减慢然后向前加速）以约1.2微米/秒的速度沿蛞蝓轴向下传播。波之间的时间间隔可能与尖端与底物接触的时间间隔有关，在正常的WS380B蛞蝓迁移过程中，每次尖端空中投射后都会周期性地重新建立这种接触。
