视网膜中视紫红质的光刺激磷酸化：存在一种对视紫红质光漂白具有特异性的蛋白激酶。
Light-stimulated phosphorylation of rhodopsin in the retina: the presence of a protein kinase that is specific for photobleached rhodopsin.
作者信息
Weller M, Virmaux N, Mandel P
出版信息
Proc Natl Acad Sci U S A. 1975 Jan;72(1):381-5. doi: 10.1073/pnas.72.1.381.
Abstract
A protein kinase has been extracted from bovine rod outer segments by a mild procedure. The enzyme acts specifically on photobleached, not unbleached, rhodopsin and will not catalyze the phosphorylation of histones, phosvitin, or casein. We propose the name "opsin kinase" for the enzyme, which is not affected by cyclic nucleotides but which is inhibited by theophylline. Preparations of purified rod outer segments, however, appear to contain only low concentration of opsin phosphatase activity.
摘要
通过温和的方法从牛视杆细胞外段提取了一种蛋白激酶。该酶特异性作用于光漂白的视紫红质，而非未漂白的视紫红质，并且不会催化组蛋白、卵黄高磷蛋白或酪蛋白的磷酸化。我们提议将该酶命名为“视蛋白激酶”，它不受环核苷酸影响，但会被茶碱抑制。然而，纯化的视杆细胞外段制剂似乎仅含有低浓度的视蛋白磷酸酶活性。
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