Tang Rong-Cai, Huang Cheng-Yin, Pei Xiao-Ping, Chen Bao-An, Li Cui-Ping, Shi Guang-Yao
Blood Center of Jiangsu Province, Nanjing 210042, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2005 Dec;13(6):1113-6.
To study the effects of glycosylation on survival of cold-storage human platelets by using rabbit model. (51)Cr-labeling platelets were used to detect the platelet storage survival. The human platelets (2.0 x 10(12)/L) treated with 5 g/L uridine diphosphate galactose (UDP-Gal) were stored in 4 degrees C refrigeratory up to 10 days. The survival of human platelets in rabbits whose reticuloendothelial system was inhibited by the administration of ethyl palmitate was monitored in blood drawn at various times after the platelet transfusion. The results showed that the survival rate of platelets was significantly increased in cold-storage human platelets by UDP-Gal treatment. The survival rates of platelets at 2 hours after transfusion into rabbits in groups of fresh platelets group, UDP-Gal + cold platelets group and cold platelets group were (68.9 +/- 8.5)%, (65.4 +/- 8.0)% and (5.0 +/- 2.6)%, respectively. Compared with cold platelets group, significant differences were seen among all groups (P < 0.01). UDP-Gal + cold platelets group had no significant differences compared with fresh platelets group (P > 0.05). It is concluded that UDG-Gal can provide the protective effect on cold-storage human platelets and prolong the survival time of refrigerated human platelets in rabbit model.
通过兔模型研究糖基化对冷藏人血小板存活的影响。采用（51）铬标记血小板检测血小板储存存活情况。将用5g/L尿苷二磷酸半乳糖（UDP-Gal）处理的人血小板（2.0×10（12）/L）在4℃冰箱中储存长达10天。在血小板输注后的不同时间采集血液，监测经棕榈酸乙酯抑制网状内皮系统的兔体内人血小板的存活情况。结果显示，UDP-Gal处理可显著提高冷藏人血小板的存活率。新鲜血小板组、UDP-Gal+冷血小板组和冷血小板组输注到兔体内2小时后的血小板存活率分别为（68.9±8.5）%、（65.4±8.0）%和（5.0±2.6）%。与冷血小板组相比，各组间差异有统计学意义（P<0.01）。UDP-Gal+冷血小板组与新鲜血小板组相比差异无统计学意义（P>0.05）。结论：UDG-Gal对冷藏人血小板具有保护作用，可延长兔模型中冷藏人血小板的存活时间。
