Yoon Jung-Hoon, Kang So-Jung, Lee Choong-Hwan, Oh Tae-Kwang
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
Int J Syst Evol Microbiol. 2006 Jan;56(Pt 1):187-91. doi: 10.1099/ijs.0.63847-0.
A Gram-negative, non-motile, non-spore-forming and rod-shaped bacterial strain, DSW-6T, was isolated from sea water and subjected to a polyphasic study. Strain DSW-6T grew optimally at 25 degrees C, in the presence of 2 % (w/v) NaCl and at pH 7.0-8.0. It was characterized chemotaxonomically as having MK-6 as the predominant menaquinone and iso-C(15 : 0), anteiso-C(15 : 0), iso-C(17 : 0) 3-OH and C(15 : 0) as the major fatty acids (>10 % of total fatty acids). Major polar lipids were phosphatidylethanolamine, unidentified phospholipids and amino-group-containing lipids that are ninhydrin-positive. The DNA G+C content was 36.9 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain DSW-6(T) forms an independent line of descent within the family Flavobacteriaceae. The 16S rRNA gene sequence of strain DSW-6T exhibited similarity values of less than 94.7 % to the sequences of other members of the family Flavobacteriaceae. Strain DSW-6T could be distinguished from other phylogenetically related genera by differences in several phenotypic properties. Therefore, in view of the combined phenotypic and phylogenetic data, it is proposed that strain DSW-6T (= KCTC 12402T = DSM 17205T) represents a novel genus and species, Donghaeana dokdonensis gen. nov., sp. nov.
从海水中分离出一株革兰氏阴性、无运动性、不形成芽孢的杆状细菌菌株DSW-6T，并对其进行了多相研究。菌株DSW-6T在25℃、2%（w/v）NaCl存在及pH 7.0 - 8.0条件下生长最佳。其化学分类特征为以MK-6作为主要甲基萘醌，以异-C(15 : 0)、anteiso-C(15 : 0)、异-C(17 : 0) 3-OH和C(15 : 0)作为主要脂肪酸（占总脂肪酸的>10%）。主要极性脂为磷脂酰乙醇胺、未鉴定的磷脂和茚三酮阳性的含氨基脂。DNA G+C含量为36.9 mol%。基于16S rRNA基因序列的系统发育分析表明，菌株DSW-6(T)在黄杆菌科内形成一个独立的进化分支。菌株DSW-6T的16S rRNA基因序列与黄杆菌科其他成员序列的相似性值小于94.7%。菌株DSW-6T可通过若干表型特征的差异与其他系统发育相关属区分开来。因此，鉴于表型和系统发育数据的综合结果，建议菌株DSW-6T（= KCTC 12402T = DSM 17205T）代表一个新的属和种，命名为东海独岛菌属（Donghaeana dokdonensis），新属，新种。
