Yoon Jung-Hoon, Kang So-Jung, Lee Mi-Hwa, Oh Tae-Kwang
Korea Research Institute of Bioscience and Biotechnology, PO Box 115, Yusong, Taejon, Republic of Korea.
Int J Syst Evol Microbiol. 2008 Aug;58(Pt 8):1892-5. doi: 10.1099/ijs.0.65704-0.
A Gram-negative, non-motile, rod-shaped, agarolytic bacterial strain, designated JW-26T, was isolated from seawater off Jeju Island in Korea, and was subjected to a polyphasic taxonomic investigation. Strain JW-26T grew optimally at pH 7.0-8.0, at 30 degrees C and in the presence of approximately 2 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain JW-26T joins Tamlana crocina HST1-43T, with which it exhibited a 16S rRNA gene sequence similarity value of 95.5 %. Strain JW-26T contained MK-6 as the predominant menaquinone and iso-C15:0, C15:0 and iso-C15:1 as the major fatty acids. The DNA G+C content was 36.8 mol%. There were no distinct phenotypic and, particularly, chemotaxonomic properties to differentiate strain JW-26T from the genus Tamlana. DNA-DNA relatedness data and differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain JW-26T is distinguishable from the type strain of T. crocina. On the basis of phenotypic, phylogenetic and genetic data, therefore, strain JW-26T represents a novel species within the genus Tamlana, for which the name Tamlana agarivorans sp. nov. is proposed. The type strain is JW-26T (=KCTC 22176T =CCUG 55327T).
从韩国济州岛附近海域分离出一株革兰氏阴性、无运动性、杆状、琼脂分解细菌菌株，命名为JW-26T，并对其进行了多相分类学研究。菌株JW-26T在pH 7.0 - 8.0、30℃以及约2%（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列的系统发育分析表明，菌株JW-26T与Tamlana crocina HST1-43T聚类，其16S rRNA基因序列相似性值为95.5%。菌株JW-26T以MK-6作为主要甲基萘醌，以异C15:0、C15:0和异C15:1作为主要脂肪酸。DNA G+C含量为36.8 mol%。没有明显的表型特征，尤其是化学分类学特征能够将菌株JW-26T与Tamlana属区分开来。DNA-DNA相关性数据和差异表型特征，以及系统发育独特性，表明菌株JW-26T与T. crocina的模式菌株不同。因此，基于表型、系统发育和遗传数据，菌株JW-26T代表Tamlana属内的一个新物种，为此提出名为Tamlana agarivorans sp. nov.的新名称。模式菌株为JW-26T（=KCTC 22176T =CCUG 55327T）。
