哈氏希瓦氏菌新种和鳕鱼希瓦氏菌新种，从波罗的海海鱼中分离得到。
Shewanella hafniensis sp. nov. and Shewanella morhuae sp. nov., isolated from marine fish of the Baltic Sea.
作者信息
Satomi Masataka, Vogel Birte Fonnesbech, Gram Lone, Venkateswaran Kasthuri
机构信息
National Research Institute of Fisheries Science, Fisheries Research Agency, Yokohama, 236-8648, Japan.
出版信息
Int J Syst Evol Microbiol. 2006 Jan;56(Pt 1):243-9. doi: 10.1099/ijs.0.63931-0.
Two novel species belonging to the genus Shewanella are described on the basis of their phenotypic characteristics, phylogenetic analyses of 16S rRNA and gyrB gene sequences and levels of DNA-DNA hybridization. A total of 47 strains belonging to two novel Gram-negative, psychrotolerant, H2S-producing bacterial species were isolated from marine fish (cod and flounder) caught from the Baltic Sea off Denmark. The phenotypic characteristics of strains belonging to group 1 (14 strains) indicated that these represented a non-sucrose-assimilating variant of Shewanella baltica with a DNA G+C content of 47.0 mol%. Strains of group 2 (33 isolates) did not utilize the carbon substrates assimilated by S. baltica except gluconate, N-acetylglucosamine and malate. Their DNA G+C content was 44.0 mol%. Phylogenetic analysis of the 16S rRNA gene sequence data placed the two novel species within the genus Shewanella. Group 1 strains showed greatest sequence similarity to Shewanella putrefaciens ATCC 8071T (99.0 %) and with S. baltica NCTC 10375(T) (98.3 %). However, gyrB gene sequence analysis showed these isolates to share only 90.0 % sequence similarity with S. putrefaciens ATCC 8071T and 93.9 % with S. baltica NCTC 10375T. Similarly, DNA-DNA hybridization experiments revealed DNA relatedness levels of 38 % between the group 1 isolates and S. putrefaciens ATCC 8071T and 43 % with S. baltica NCTC 10375T. The group 2 strains shared less than 97 % 16S rRNA gene sequence similarities with recognized Shewanella species. Comparisons between the two novel species indicated 16S rRNA gene sequence similarity of approximately 98 %, gyrB gene sequence similarity of approximately 89 % and DNA-DNA reassociation values of 20-34 %. Based on the evidence presented, two novel species, Shewanella hafniensis sp. nov. (type strain P010T = ATCC BAA-1207T = NBRC 100975T) and Shewanella morhuae sp. nov. (type strain U1417T = ATCC BAA-1205T = NBRC 100978T), are described.
基于表型特征、16S rRNA和gyrB基因序列的系统发育分析以及DNA-DNA杂交水平，描述了希瓦氏菌属的两个新物种。从丹麦附近波罗的海捕获的海鱼（鳕鱼和比目鱼）中分离出总共47株属于两个新的革兰氏阴性、耐冷、产H2S细菌物种的菌株。属于第1组的菌株（14株）的表型特征表明，这些菌株代表了波罗的海希瓦氏菌的一个不利用蔗糖的变体，其DNA G+C含量为47.0 mol%。第2组的菌株（33株分离株）除葡萄糖酸盐、N-乙酰葡糖胺和苹果酸盐外，不利用波罗的海希瓦氏菌利用的碳底物。它们的DNA G+C含量为44.0 mol%。16S rRNA基因序列数据的系统发育分析将这两个新物种置于希瓦氏菌属内。第1组菌株与腐败希瓦氏菌ATCC 8071T（99.0%）和波罗的海希瓦氏菌NCTC 10375(T)（98.3%）显示出最大的序列相似性。然而，gyrB基因序列分析表明，这些分离株与腐败希瓦氏菌ATCC 8071T的序列相似性仅为90.0%，与波罗的海希瓦氏菌NCTC 10375T的序列相似性为93.9%。同样，DNA-DNA杂交实验显示，第1组分离株与腐败希瓦氏菌ATCC 8071T的DNA相关性水平为38%，与波罗的海希瓦氏菌NCTC 10375T的DNA相关性水平为43%。第2组菌株与已确认的希瓦氏菌物种的16S rRNA基因序列相似性低于97%。两个新物种之间的比较表明，16S rRNA基因序列相似性约为98%，gyrB基因序列相似性约为89%，DNA-DNA重结合值为20-34%。根据所提供的证据，描述了两个新物种，即哈氏希瓦氏菌（Shewanella hafniensis sp. nov.）（模式菌株P010T = ATCC BAA-1207T = NBRC 100975T）和鳕鱼希瓦氏菌（Shewanella morhuae sp. nov.）（模式菌株U1417T = ATCC BAA-1205T = NBRC 100978T）。
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