Satomi Masataka, Vogel Birte Fonnesbech, Venkateswaran Kasthuri, Gram Lone
National Research Institute of Fisheries Science, Fisheries Research Agency, 2-12-4 Fukuura, Kanazawa-ku, Yokohama 236-8648, Japan.
Danish Institute for Fisheries Research, Department of Seafood Research, Søltofts Plads, c/o Technical University of Denmark, Bldg 221, DK-2800 Kgs Lyngby, Denmark.
Int J Syst Evol Microbiol. 2007 Feb;57(Pt 2):347-352. doi: 10.1099/ijs.0.64708-0.
Two novel species belonging to the genus Shewanella are described on the basis of a polyphasic taxonomic approach. A total of 40 strains of Gram-negative, psychrotolerant, H2S-producing bacteria were isolated from marine fish (cod and plaice) caught in the Baltic Sea off Denmark. Strains belonging to group 1 (seven strains) were a lactate-assimilating variant of Shewanella morhuae with a G+C content of 44 mol%. The strains of group 2 (33 strains) utilized lactate, N-acetylglucosamine and malate but did not produce DNase or ornithine decarboxylase. Their G+C content was 47 mol%. Phylogenetic analysis of the 16S rRNA gene sequence data placed the two novel species within the genus Shewanella. Group 1 showed greatest sequence similarity with S. morhuae ATCC BAA-1205T (99.9 %). However, gyrB gene sequence analysis and DNA-DNA hybridization differentiated these isolates from S. morhuae, with 95.6 % sequence similarity and less than 57 % DNA relatedness, respectively. Group 2 strains shared more than 99 % 16S rRNA gene sequence similarity with the type strains of Shewanella colwelliana and Shewanella affinis, but gyrB sequence similarity ( approximately 85 %) and the results of DNA hybridization ( approximately 28 %) indicated that the new isolates represented a novel species. Furthermore, when compared to each other, the type strains of S. colwelliana and S. affinis had almost identical gyrB sequences and significantly high DNA reassociation values (76-83 %), indicating that they belonged to the same species. Based on the conclusions of this study, we propose the novel species Shewanella glacialipiscicola sp. nov. (type strain T147T=LMG 23744T=NBRC 102030T) for group 1 strains and Shewanella algidipiscicola sp. nov. (type strain S13T=LMG 23746T=NBRC 102032T) for group 2 strains, and we propose that Shewanella affinis as a later heterotypic synonym of Shewanella colwelliana.
基于多相分类学方法，描述了希瓦氏菌属的两个新物种。从丹麦附近波罗的海捕获的海鱼（鳕鱼和鲽鱼）中总共分离出40株革兰氏阴性、耐冷、产H2S的细菌。属于第1组的菌株（7株）是莫氏希瓦氏菌的乳酸同化变体，G+C含量为44 mol%。第2组的菌株（33株）利用乳酸、N-乙酰葡糖胺和苹果酸，但不产生DNA酶或鸟氨酸脱羧酶。它们的G+C含量为47 mol%。对16S rRNA基因序列数据的系统发育分析将这两个新物种置于希瓦氏菌属内。第1组与莫氏希瓦氏菌ATCC BAA-1205T的序列相似性最高（99.9%）。然而，gyrB基因序列分析和DNA-DNA杂交将这些分离株与莫氏希瓦氏菌区分开来，序列相似性分别为95.6%，DNA相关性小于57%。第2组菌株与科尔韦氏希瓦氏菌和近缘希瓦氏菌的模式菌株共享超过99%的16S rRNA基因序列相似性，但gyrB序列相似性（约85%）和DNA杂交结果（约28%）表明新分离株代表一个新物种。此外，相互比较时，科尔韦氏希瓦氏菌和近缘希瓦氏菌的模式菌株具有几乎相同的gyrB序列和显著高的DNA重结合值（76-83%），表明它们属于同一物种。基于本研究的结论，我们为第1组菌株提出新物种冰川鱼希瓦氏菌（Shewanella glacialipiscicola sp. nov.）（模式菌株T147T = LMG 23744T = NBRC 102030T），为第2组菌株提出新物种冷海鱼希瓦氏菌（Shewanella algidipiscicola sp. nov.）（模式菌株S13T = LMG 23746T = NBRC 102032T），并且我们提议将近缘希瓦氏菌作为科尔韦氏希瓦氏菌的晚出异名。
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