Lud Simon Q, Nikolaides Michael G, Haase Ilka, Fischer Markus, Bausch Andreas R
Lehrstuhl für Biophysik--E22, Technische Universität München, James Franck Str. 1, 85747 Garching (Germany).
Chemphyschem. 2006 Feb 13;7(2):379-84. doi: 10.1002/cphc.200500484.
For many biotechnological applications the label-free detection of biomolecular interactions is becoming of outstanding importance. In this Article we report the direct electrical detection of small peptides and proteins by their intrinsic charges using a biofunctionalized thin-film resistor. The label-free selective and quantitative detection of small peptides and proteins is achieved using hydrophobized silicon-on-insulator (SOI) substrates functionalized with lipid membranes that incorporate metal-chelating lipids. The response of the nanometer-thin conducting silicon film to electrolyte screening effects is taken into account to determine quantitatively the charges of peptides. It is even possible to detect peptides with a single charge and to distinguish single charge variations of the analytes even in physiological electrolyte solutions. As the device is based on standard semiconductor technologies, parallelization and miniaturization of the SOI-based biosensor is achievable by standard CMOS technologies and thus a promising basis for high-throughput screening or biotechnological applications.
对于许多生物技术应用而言，生物分子相互作用的无标记检测正变得极为重要。在本文中，我们报告了使用生物功能化薄膜电阻器通过小肽和蛋白质的固有电荷对其进行直接电学检测。通过使用疏水化的绝缘体上硅（SOI）衬底实现小肽和蛋白质的无标记选择性和定量检测，该衬底用掺入金属螯合脂质的脂质膜进行功能化。考虑到纳米级导电硅膜对电解质屏蔽效应的响应，以定量确定肽的电荷。甚至有可能检测带单个电荷的肽，并且即使在生理电解质溶液中也能区分分析物的单电荷变化。由于该器件基于标准半导体技术，基于SOI的生物传感器的并行化和小型化可通过标准CMOS技术实现，因此是高通量筛选或生物技术应用的有前景的基础。
