Department of Physics, Faculty of Sciences and Technology, UIN Syarif Hidayatullah Jakarta, Tangerang Selatan, Banten 15412, Indonesia.
Department of Physics, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, Padang 25131, Indonesia.
Biosensors (Basel). 2018 Jan 30;8(1):12. doi: 10.3390/bios8010012.
A polyvinylidene fluoride (PVDF) film-based capacitive biosensor was developed for glucose sensing. This device consists of a PVDF film sandwiched between two electrodes. A capacitive biosensor measures the dielectric properties of the dielectric layers at the interface between the electrolyte and the electrode. A glucose oxidase (GOx) enzyme was immobilized onto the electrode to oxidize glucose. In practice, the biochemical reaction of glucose with the GOx enzyme generates free electron carriers. Consequently, the potential difference between the electrodes is increased, resulting in a measurable voltage output of the biosensor. The device was tested for various glucose concentrations in the range of 0.013 to 5.85 M, and various GOx enzyme concentrations between 4882.8 and 2.5 million units/L. We found that the sensor output increased with increasing glucose concentration up to 5.85 M. These results indicate that the PVDF film-based capacitive biosensors can be properly applied to glucose sensing and provide opportunities for the low-cost fabrication of glucose-based biosensors based on PVDF materials.
一种基于聚偏二氟乙烯（PVDF）薄膜的电容式生物传感器被开发用于葡萄糖传感。该器件由夹在两个电极之间的 PVDF 薄膜组成。电容式生物传感器测量电解质和电极之间界面处电介质层的介电特性。葡萄糖氧化酶（GOx）酶被固定在电极上以氧化葡萄糖。实际上，葡萄糖与 GOx 酶的生化反应会产生游离电子载体。因此，电极之间的电位差增加，导致生物传感器可测量的电压输出。该器件在 0.013 至 5.85 M 的各种葡萄糖浓度以及 4882.8 至 250 万单位/L 的各种 GOx 酶浓度下进行了测试。我们发现传感器输出随葡萄糖浓度的增加而增加，最高可达 5.85 M。这些结果表明，基于 PVDF 薄膜的电容式生物传感器可适当地用于葡萄糖传感，并为基于 PVDF 材料的低成本葡萄糖生物传感器制造提供机会。
