Vogiatzidis Konstantinos, Hatzoglou Chrissi, Zarogiannis Sotirios, Matafia Galatia, Gourgoulianis Konstantinos, Molyvdas Paschalis-Adam
Department of Physiology, Medical School, University of Thessaly, 22 Papakiriazi Str, 41222, Larissa, Greece.
Eur J Pharmacol. 2006 Jan 20;530(3):276-80. doi: 10.1016/j.ejphar.2005.11.050. Epub 2006 Jan 5.
The effect of morphine (mu-opioid receptor agonist) on the transmesothelial resistance (R(TM)) of sheep's pleura and parietal pericardium was studied using the Ussing chamber technique. Basal transmesothelial resistance of parietal pleura was found to be 19.57+/-0.32 Omega cm2 and of visceral pleura was found to be 19.41+/-0.31 Omega cm2, whereas that of parietal pericardium was found to be 22.83+/-0.4 Omega cm2. Immediately after the addition of morphine (10(-9) M) both apically and basolaterally on the parietal pleura and parietal pericardium, these values were significantly increased (P<0.05). On the contrary, addition of morphine (10(-9) M) resulted in a rapid increase, only when placed basolaterally on the visceral pleura (P<0.05). In conclusion, our findings suggest that morphine, probably through mu-opioid stimulation, increases in vitro the transmesothelial resistance of the parietal pleura, of the visceral pleura when added basolaterally and of the parietal pericardium.
使用尤斯灌流小室技术研究了吗啡（μ-阿片受体激动剂）对绵羊胸膜和壁层心包跨间皮电阻（R(TM)）的影响。发现壁层胸膜的基础跨间皮电阻为19.57±0.32Ω·cm2，脏层胸膜为19.41±0.31Ω·cm2，而壁层心包的基础跨间皮电阻为22.83±0.4Ω·cm2。在壁层胸膜和壁层心包的顶端和基底外侧即刻添加吗啡（10(-9)M）后，这些值显著升高（P<0.05）。相反，仅当在脏层胸膜的基底外侧放置吗啡（10(-9)M）时，会导致快速升高（P<0.05）。总之，我们的研究结果表明，吗啡可能通过μ-阿片刺激，在体外增加壁层胸膜、基底外侧添加吗啡时的脏层胸膜以及壁层心包的跨间皮电阻。
