Germanà A, Montalbano G, de Carlos F, Levanti M B, Abbate F, Vega J A, Ciriaco E
Dipartimento di Morfologia, Biochimica, Fisiologia e Produzione Animale, Sezione di Morfologia, Università di Messina, Polo Universitario Annunziata, 98168 Messina, Italy.
Neurosci Lett. 2006 Apr 24;397(3):210-3. doi: 10.1016/j.neulet.2005.12.015. Epub 2006 Jan 6.
The mechano and chemosensory organs of adult teleosts undergoes a continuous cell renewal and turnover which is regulated in part by growth factors. Here, we investigated the occurrence and the cell localization of epidermal growth factor (EGF) in the lateral line system and taste bud of adult zebrafish, using Western blot and immunohistochemistry associated to a polyclonal antibody against mammalian EGF. Furthermore, the distribution of S100 protein was studied in parallel to label hair sensory cells in the lateral line system. Western blot revealed one unique protein band with an estimated molecular weight of about 13 kDa, equivalent to the EGF of mammals. Specific immunoreactivity for EGF was observed in the epithelial basal and/or supporting cells of the neuromasts of the lateral line system and taste buds. Conversely, the sensory cells in both sensory structures were devoid of immunostaining. Present results demonstrate the occurrence of EGF in mechano and sensory system of adult zebrafish, suggesting a role for this molecule in the cell renewal and turnover of these structures.
成年硬骨鱼的机械感觉和化学感觉器官会经历持续的细胞更新和更替，这部分受生长因子调节。在此，我们使用与抗哺乳动物表皮生长因子（EGF）的多克隆抗体相关的蛋白质免疫印迹法和免疫组织化学方法，研究了成年斑马鱼侧线系统和味蕾中表皮生长因子（EGF）的存在情况及其细胞定位。此外，平行研究了S100蛋白的分布，以标记侧线系统中的毛感觉细胞。蛋白质免疫印迹法显示出一条独特的蛋白带，估计分子量约为13 kDa，与哺乳动物的EGF相当。在侧线系统神经丘和味蕾的上皮基底细胞和/或支持细胞中观察到EGF的特异性免疫反应性。相反，两种感觉结构中的感觉细胞均无免疫染色。目前的结果证明了成年斑马鱼的机械感觉和感觉系统中存在EGF，表明该分子在这些结构的细胞更新和更替中发挥作用。
