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BDNF 和 Calretinin 在斑马鱼嗅上皮和味蕾中的定位（）。

Localization of BDNF and Calretinin in Olfactory Epithelium and Taste Buds of Zebrafish ().

机构信息

Zebrafish Neuromorphology Lab, Department of Veterinary Sciences, University of Messina, 98168 Messina, Italy.

Departamento de Cirugía y Especialidades Médico-Quirúrgicas, Universidad de Oviedo, 33006 Oviedo, Spain.

出版信息

Int J Mol Sci. 2022 Apr 23;23(9):4696. doi: 10.3390/ijms23094696.

DOI:10.3390/ijms23094696
PMID:35563087
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9101965/
Abstract

Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family and it is involved in several fundamental functions in the central and peripheral nervous systems, and in sensory organs. BDNF regulates the chemosensory systems of mammals and is consistently expressed in those organs. In zebrafish, the key role of BDNF in the biology of the hair cells of the inner ear and lateral line system has recently been demonstrated. However, only some information is available about its occurrence in the olfactory epithelium, taste buds, and cutaneous isolated chemosensory cells. Therefore, this study was undertaken to analyze the involvement of BDNF in the chemosensory organs of zebrafish during the larval and adult stages. To identify cells displaying BDNF, we compared the cellular pattern of BDNF-displaying cells with those immunoreactive for calretinin and S100 protein. Our results demonstrate the localization of BDNF in the sensory part of the olfactory epithelium, mainly in the ciliated olfactory sensory neurons in larvae and adult zebrafish. Intense immunoreaction for BDNF was also observed in the chemosensory cells of oral and cutaneous taste buds. Moreover, a subpopulation of olfactory sensory neurons and chemosensory cells of olfactory rosette and taste bud, respectively, showed marked immunopositivity for calcium-binding protein S100 and calretinin. These results demonstrate the possible role of BDNF in the development and maintenance of olfactory sensory neurons and sensory cells in the olfactory epithelium and taste organs of zebrafish during all stages of development.

摘要

脑源性神经营养因子（BDNF）是神经营养因子家族的一员，它参与中枢和外周神经系统以及感觉器官的几种基本功能。BDNF 调节哺乳动物的化学感觉系统，并在这些器官中持续表达。在斑马鱼中，BDNF 在内耳毛细胞和侧线系统的生物学中的关键作用最近已经得到证明。然而，关于其在内耳嗅上皮、味蕾和皮肤分离化学感觉细胞中的存在，只有一些信息。因此，本研究旨在分析 BDNF 在幼鱼和成年斑马鱼的化学感觉器官中的作用。为了鉴定显示 BDNF 的细胞，我们将显示 BDNF 的细胞的细胞模式与对 calretinin 和 S100 蛋白呈免疫反应的细胞进行比较。我们的结果表明 BDNF 定位于嗅上皮的感觉部分，主要位于幼鱼和成年斑马鱼的纤毛嗅感觉神经元中。BDNF 的强烈免疫反应也在口腔和皮肤味蕾的化学感觉细胞中观察到。此外，嗅上皮和味蕾的嗅轮和味蕾的化学感觉细胞中的嗅感觉神经元和化学感觉细胞的亚群分别对钙结合蛋白 S100 和 calretinin 表现出明显的免疫阳性。这些结果表明 BDNF 在发育过程中的所有阶段都可能在嗅觉感觉神经元和嗅觉上皮和味觉器官中的感觉细胞的发育和维持中发挥作用。

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