Tewtrakul Supinya, Itharat Arunporn, Rattanasuwan Pranee
Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand.
J Ethnopharmacol. 2006 Apr 21;105(1-2):312-5. doi: 10.1016/j.jep.2005.11.021. Epub 2006 Jan 6.
Ethanolic- and water extracts from five species of Thai medicinal plants known as Hua-Khao-Yen were tested for their inhibitory effects against HIV-1 protease (HIV-PR) and HIV-1 integrase (HIV-1 IN). The result revealed that the ethanolic (EtOH) extract of Smilax corbularia exhibited anti-HIV-1 IN activity with an IC50 value of 1.9 microg/ml, followed by the water extract of Dioscorea birmanica (IC50 = 4.5 microg/ml), the EtOH extract of Dioscorea birmanica (IC50 = 4.7 microg/ml), the water extract of Smilax corbularia (IC50 = 5.4 microg/ml), the EtOH extract of Smilax glabra (IC50 = 6.7 microg/ml) and the water extract of Smilax glabra (IC50 = 8.5 microg/ml). The extracts of Pygmaeopremna herbacea and Dioscorea membranacea were apparently inactive (IC50 > 100 microg/ml). Interestingly, only the EtOH extract of Dioscorea membranacea showed appreciable activity (IC50 = 48 microg/ml) against HIV-1 PR, while the other extracts possessed mild activity. This result strongly supported the basis for the use of Smilax corbularia and Dioscorea membranacea for AIDS treatment by Thai traditional doctors.
对五种名为华考延的泰国药用植物的乙醇提取物和水提取物进行了抗HIV-1蛋白酶（HIV-PR）和HIV-1整合酶（HIV-1 IN）抑制作用的测试。结果显示，牛尾菜乙醇提取物表现出抗HIV-1 IN活性，IC50值为1.9微克/毫升，其次是滇薯蓣水提取物（IC50 = 4.5微克/毫升）、滇薯蓣乙醇提取物（IC50 = 4.7微克/毫升）、牛尾菜水提取物（IC50 = 5.4微克/毫升）、光叶菝葜乙醇提取物（IC50 = 6.7微克/毫升）和光叶菝葜水提取物（IC50 = 8.5微克/毫升）。矮小豆腐柴和膜叶薯蓣的提取物明显无活性（IC50 > 100微克/毫升）。有趣的是，只有膜叶薯蓣乙醇提取物对HIV-1 PR表现出可观的活性（IC50 = 48微克/毫升），而其他提取物活性较弱。这一结果有力地支持了泰国传统医生使用牛尾菜和膜叶薯蓣治疗艾滋病的依据。
