Pedernera Ana María, Guardia Teresita, Calderón Carola Guardia, Rotelli Alejandra Ester, de la Rocha Nadir Ernesto, Genaro Silvia Di, Pelzer Lilian Eugenia
Cátedra de Farmacología, Departamento de Farmacia, Facultad de Química, Bioquímica y Farmacia, Chacabuco y Pedernera, 5700 San Luis, Argentina.
J Ethnopharmacol. 2006 May 24;105(3):415-20. doi: 10.1016/j.jep.2005.11.016. Epub 2006 Jan 6.
Larrea divaricata Cav. (Zygophyllaceae) is used in popular medicine as anti-inflammatory and anti-rheumatic drug, containing higher amounts of nordihydroguaiaretic acid, a well-known free radical scavenger. Here we describe the gastric anti-ulcerogenic effect of the methanolic extract of Larrea divaricata (LdME) leaves in rat, using absolute ethanol and HCl 0.6N as necrotizing agents, and the effect of blocking endogenous sulfhydryl (SH) groups with N-ethylmaleimide (NEM). We also studied the anti-inflammatory activity of LdME in two rat experimental models: cotton pellet induced granuloma and arthritis induced by adjuvant-carrageenan. Influence of treatment in body weight and some lymphoid organs was evaluated. Finally, the free radical scavenging capacity of LdME was determined by the method of 1,1-diphenyl-2-picrylhydrazyl (DPPH) inhibition. LdME demonstrated anti-ulcerogenic effect in rats, and it was shown that the endogenous SH compounds were not involved in the mechanism of action. LdME also showed inhibitory activity in the applied models of inflammation, being more effective in the acute phase. No effect was observed concerning the weight of spleen, thymus and body. The extract proved to possess elevated free radical scavenging capacity which may contribute to the observed gastric anti-ulcerogenic and anti-inflammatory activity.
拉瑞阿草（Larrea divaricata Cav.，蒺藜科）在民间医学中用作抗炎和抗风湿药物，含有较高含量的去氢二松柏醇酸，这是一种著名的自由基清除剂。在此，我们描述了拉瑞阿草叶甲醇提取物（LdME）对大鼠的胃抗溃疡作用，使用无水乙醇和0.6N盐酸作为坏死剂，以及用N - 乙基马来酰亚胺（NEM）阻断内源性巯基（SH）基团的作用。我们还在两种大鼠实验模型中研究了LdME的抗炎活性：棉球诱导的肉芽肿和佐剂 - 角叉菜胶诱导的关节炎。评估了治疗对体重和一些淋巴器官的影响。最后，通过1,1 - 二苯基 - 2 - 苦基肼（DPPH）抑制法测定了LdME的自由基清除能力。LdME在大鼠中表现出抗溃疡作用，并且表明内源性SH化合物不参与其作用机制。LdME在应用的炎症模型中也显示出抑制活性，在急性期更有效。未观察到对脾脏、胸腺重量和体重的影响。提取物被证明具有较高的自由基清除能力，这可能有助于观察到的胃抗溃疡和抗炎活性。
