Fang Qing Mao, Zhang Hao, Cao Yu, Wang Can
West China School of Pharmacy, Sichuan University, No. 17, Section 3, Ren-min-nan-lu Road, Chengdu, Sichuan 610041, PR China.
J Ethnopharmacol. 2007 Oct 8;114(1):61-5. doi: 10.1016/j.jep.2007.07.024. Epub 2007 Jul 31.
The seeds of Herpetospermum pedunculosun (Ser.) C.B.Clarke. (HPD), Mormodica cochinchinensis (Lour.) Spreng. (MCC) and Mormodica charantia L. (MCR) share the same common name ("Bolengguazi") in the Tibetan medicine. The anti-inflammatory activities of the three "Bolengguazi" were evaluated using egg album-induced paw edema and cotton-pellet granuloma tests. Among them, HPD is the most active one in both models. Results showed that oral administration of ethanol extract of HPD seed (200 and 400mg/kg) significantly suppressed the development of egg albumin-induced paw edema (P<0.01). In the chronic test, the ethanol extract of HPD seed (200 and 400mg/kg) showed significant reduction in granuloma weight of rats (P<0.05). The anti-inflammatory effect of the ethanol HPD seed was similar to that of indomethacin (10mg/kg). Moreover, the free radical scavenging activities of "Bolengguazi" were investigated using the DPPH test. Only the ethanol extract of HPD seed showed a moderate free radical scavenging effect on DPPH (IC50, 198.69 microg/ml) when compared with the positive control, V(E), (IC50, 44.91 microg/ml). The HPD seeds showed more significant anti-inflammatory activities in additional to higher free radical scavenging activities than that of the MCC and MCR seeds. The results of the present study provide a scientific basis to explain, in part, the popular use of HPD seed in Tibetan folk medicine as "Bolengguazi". The present study also supports the claims by the traditional Tibetan medicine practitioners about the use of HPD seeds in inflammatory diseases, such as "Chiba".
藏药中，波棱瓜（Herpetospermum pedunculosun (Ser.) C.B.Clarke.，简称HPD）、木鳖子（Mormodica cochinchinensis (Lour.) Spreng.，简称MCC）和罗汉果（Mormodica charantia L.，简称MCR）的种子有着相同的俗名（“波棱瓜子”）。采用蛋清致足肿胀和棉球肉芽肿试验对这三种“波棱瓜子”的抗炎活性进行了评价。其中，HPD在两种模型中活性最强。结果表明，口服HPD种子乙醇提取物（200和400mg/kg）能显著抑制蛋清诱导的足肿胀（P<0.01）。在慢性试验中，HPD种子乙醇提取物（200和400mg/kg）能显著降低大鼠肉芽肿重量（P<0.05）。HPD种子乙醇提取物的抗炎作用与吲哚美辛（10mg/kg）相似。此外，采用DPPH试验研究了“波棱瓜子”的自由基清除活性。与阳性对照维生素E（IC50，44.91μg/ml）相比，只有HPD种子乙醇提取物对DPPH有中等程度的自由基清除作用（IC50，198.69μg/ml）。与MCC和MCR种子相比，HPD种子不仅具有更强的自由基清除活性，还具有更显著的抗炎活性。本研究结果为部分解释HPD种子在藏药中作为“波棱瓜子”的广泛应用提供了科学依据。本研究还支持了传统藏医关于HPD种子用于治疗诸如“赤巴”等炎症性疾病的说法。
